De Jong M H, Van der Hoeven J S, Van Os J H
J Dent Res. 1986 Feb;65(2):85-8. doi: 10.1177/00220345860650021601.
The role of saliva in supporting the growth of dental plaque has scarcely been investigated. We have studied the growth and recovery of micro-organisms from dental plaque samples on saliva-agar plates, prepared from filter-sterilized wax-stimulated whole saliva. Under optimal conditions, the mean recovery of plaque samples on saliva agar was about 50% (range, 22-77) of the recovery on blood agar. Addition of 2.5 mmol/L dithiothreitol (DTT) strongly facilitated filter-sterilization of saliva, but DTT concentrations higher than 1 mmol/L reduced the recovery of plaque micro-organisms on saliva agar. Catalase (100 U/ml) offered protection against the inhibitory effect of DTT. Addition of glucose (1-5 mmol/L) increased only slightly the recovery of plaque micro-organisms on saliva agar. When supragingival plaque was plated on saliva- and blood-agar plates, the composition of the microflora isolated from saliva agar strongly resembled that isolated from blood agar. The predominant species--i.e., Streptococcus and Actinomyces--all grew on saliva agar though usually in numbers somewhat lower than those on blood agar. In addition, most species normally found only in low proportions in supragingival dental plaque were also found to grow on saliva agar. Collectively, the results lead to the conclusion that the supragingival microflora can utilize saliva as a complete source of nutrients.
唾液在支持牙菌斑生长方面的作用几乎未被研究过。我们研究了从用滤菌法处理的石蜡刺激全唾液制备的唾液琼脂平板上的牙菌斑样本中微生物的生长和复苏情况。在最佳条件下，唾液琼脂上牙菌斑样本的平均复苏率约为血琼脂上复苏率的50%（范围为22%-77%）。添加2.5 mmol/L二硫苏糖醇（DTT）极大地促进了唾液的滤菌处理，但DTT浓度高于1 mmol/L会降低唾液琼脂上牙菌斑微生物的复苏率。过氧化氢酶（100 U/ml）可抵御DTT的抑制作用。添加葡萄糖（1-5 mmol/L）仅略微提高了唾液琼脂上牙菌斑微生物的复苏率。当将龈上菌斑接种在唾液琼脂平板和血琼脂平板上时，从唾液琼脂上分离出的微生物群落组成与从血琼脂上分离出的极为相似。主要菌种，即链球菌和放线菌，都能在唾液琼脂上生长，不过其数量通常略低于在血琼脂上的数量。此外，通常在龈上牙菌斑中仅占低比例的大多数菌种也被发现能在唾液琼脂上生长。总体而言，这些结果得出结论：龈上微生物群落可将唾液作为完整的营养来源。
