Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748, Olsztyn, Poland.
Polish Academy of Sciences, Institute of Ichthyobiology and Aquaculture in Gołysz, Zaborze, 43-520, Chybie, Poland.
Fish Shellfish Immunol. 2021 Nov;118:354-368. doi: 10.1016/j.fsi.2021.09.018. Epub 2021 Sep 22.
In carp aquaculture, hormonal manipulation with an analog of GnRH (Ovopel) and carp pituitary extract (CPE), which act at different levels of the hypothalamic-pituitary-gonadal axis, is a routine practice to enhance sperm production. Our recent studies revealed that hormonal stimulation of male carp was associated with changes in the seminal plasma proteome, including blood origin proteins. Here, we explored whether Ovopel and CPE could affect the blood proteome of male carp. Both preparations induced increases in semen volume, total number of sperm, and testosterone level. However, hormonal stimulation did not affect the plasma cortisol and glucose levels. A comparative proteomic analysis of carp blood plasma between the control (PBS) and the hormonally treated males revealed significant changes (>1.2 <-1.2-fold change, P < 0.05) in the abundance of 30 spots (14 up- and 16 downregulated) and 44 spots (28 up- and 16 downregulated) upon CPE and Ovopel treatment, respectively. The most significantly affected pathways were acute phase response signaling, the coagulation system, LXR/RXR and FXR/RXR activation; however, there were different sets of proteins in Ovopel- and CPE-treated males. The majority of differentially abundant proteins were involved in the regulation of the immune defense response, the response to stress, and complement activation. Moreover hormonal stimulation with CPE markedly increased the bactericidal activity of blood and both preparations caused profound changes in gene expression in hematopoietic organs. This work is important in understanding the biological processes behind the protein-based response to hormonal stimulation of sperm production in fish.
在鲤鱼养殖中，使用 GnRH 类似物（Ovopel）和鲤鱼脑垂体提取物（CPE）对鱼类进行荷尔蒙处理，这两种物质在下丘脑-垂体-性腺轴的不同水平起作用，是提高精子产量的常规做法。我们最近的研究表明，雄性鲤鱼的荷尔蒙刺激与精液蛋白质组的变化有关，包括血液来源的蛋白质。在这里，我们探讨了 Ovopel 和 CPE 是否会影响雄性鲤鱼的血液蛋白质组。这两种制剂都能增加精液量、精子总数和睾酮水平。然而，荷尔蒙刺激并没有影响血浆皮质醇和葡萄糖水平。通过对对照组（PBS）和经荷尔蒙处理的雄性鲤鱼的血浆进行比较蛋白质组学分析，发现 CPE 和 Ovopel 处理后，分别有 30 个（上调 14 个，下调 16 个）和 44 个（上调 28 个，下调 16 个）斑点的丰度发生显著变化（变化倍数>1.2 或<-1.2，P<0.05）。受影响最显著的途径是急性期反应信号转导、凝血系统、LXR/RXR 和 FXR/RXR 激活；然而，在 CPE 和 Ovopel 处理的雄性中，存在不同的蛋白质组。差异丰度最大的蛋白质大多参与免疫防御反应的调节、应激反应和补体激活。此外，CPE 对血液的杀菌活性有明显的增强作用，这两种制剂还导致造血器官基因表达的深刻变化。这项工作对于理解鱼类精子产生的基于蛋白质的对荷尔蒙刺激的反应背后的生物学过程具有重要意义。
