Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748, Olsztyn, Poland.
Fish Disease Research Unit, Institute for Parasitology, University of Veterinary Medicine Hannover, Buenteweg 17, 30559, Hannover, Germany.
Fish Shellfish Immunol. 2022 Aug;127:822-835. doi: 10.1016/j.fsi.2022.07.019. Epub 2022 Jul 15.
Aeromonas salmonicida is recognized as a significant bacterial pathogen in ulcerative disease of cyprinid fish. However, the mechanism of immunity to these bacteria in common carp is still not well understood, especially the immune regulation in the gonad to bacterial infection. The aims of our study were to analyze changes in the seminal plasma proteome following A. salmonicida infection in carp males. The observed pathological changes in the tissue (liver, spleen, kidney and testis) morphology and upregulation of immune-related genes (tnfa2, il6a) confirmed the successful infection challenge. Using mass spectrometry-based label-free quantitative proteomics, we identified 1402 seminal plasma proteins, and 44 proteins (20 up- and 24 downregulated) were found to be differentially abundant between infected and control males. Most differentially abundant proteins were involved in the immune response mechanisms, such as acute phase response, complement activation and coagulation, inflammation, lipid metabolism, cell-cell and cell-matrix adhesion, creatine-phosphate biosynthesis and germ cell-Sertoli cell junction signaling. Bacterial infection also caused profound changes in expression of selected genes in the testis and hematopoietic organs, which contributed to changes in seminal proteins. The altered seminal proteins and bacterial proteins in seminal plasma may serve as valuable markers of infection in the testis.
气单胞菌被认为是鲤鱼溃疡性疾病的重要细菌性病原体。然而，鲤鱼对这些细菌的免疫机制仍不清楚，特别是在性腺对细菌感染的免疫调节方面。我们的研究目的是分析感染后雄性鲤鱼精液蛋白组的变化。组织（肝、脾、肾和睾丸）形态的观察到的病理变化和免疫相关基因（tnfa2，il6a）的上调证实了成功的感染挑战。使用基于质谱的无标记定量蛋白质组学，我们鉴定了 1402 种精液蛋白，其中 44 种蛋白（20 种上调和 24 种下调）在感染和对照雄性之间存在差异丰度。大多数差异丰度蛋白参与免疫反应机制，如急性期反应、补体激活和凝血、炎症、脂质代谢、细胞-细胞和细胞-基质黏附、磷酸肌酸生物合成和生殖细胞-支持细胞连接信号。细菌感染还导致睾丸和造血器官中选定基因的表达发生深刻变化，这导致精液蛋白发生变化。精液中改变的精液蛋白和细菌蛋白可能作为睾丸感染的有价值标志物。
