College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Int J Mol Sci. 2023 Oct 14;24(20):15171. doi: 10.3390/ijms242015171.
Vascular endothelial growth factor A (VEGFA) plays important roles in angiogenesis, inflammatory response as well as energy metabolism in mammals. However, its effect on glycolipid metabolism in fish has not been reported. In this study, we cloned and characterized the gene of . expression was significantly higher in liver and muscle than that in other tissues. Then, the VEGFA recombinant protein was expressed in and obtained after purification. VEGFA i.p. injection significantly increased the serum glucose and TG content compared with the control group. Moreover, VEGFA protein aggravated the glycogen and lipid deposition in the liver of . In addition, we found that VEGFA treatment increased hepatocyte glycogen and lipid droplet content and increased the levels of pAMPKα (T172). Furthermore, AMPKα inhibition attenuated the ability of VEGFA to induce TG and glycogen accumulation. These results demonstrate that VEGFA regulates hepatic lipid and glycogen metabolism through AMPKα in , which may contribute to a better understanding of VEGFA functions in the glycolipid metabolism of fish.
血管内皮生长因子 A（VEGFA）在哺乳动物的血管生成、炎症反应以及能量代谢中发挥着重要作用。然而，其在鱼类糖脂代谢中的作用尚未见报道。在本研究中，我们克隆并鉴定了 基因。其在肝脏和肌肉中的表达显著高于其他组织。随后，我们在 中表达并纯化了 VEGFA 重组蛋白。与对照组相比，VEGFA 腹腔注射显著增加了血清葡萄糖和甘油三酯的含量。此外，VEGFA 蛋白加重了 肝脏中的糖原和脂质沉积。另外，我们发现 VEGFA 处理增加了肝细胞的糖原和脂滴含量，并提高了 pAMPKα（T172）的水平。此外，AMPKα 抑制减弱了 VEGFA 诱导甘油三酯和糖原积累的能力。这些结果表明，VEGFA 通过 中的 AMPKα 调节肝脏的脂质和糖原代谢，这可能有助于更好地理解 VEGFA 在鱼类糖脂代谢中的功能。
