Technical University of Darmstadt, Darmstadt, Germany.
Institute of Virology, Vaccines and Sera - TORLAK, Belgrade, Serbia.
J Photochem Photobiol B. 2021 Nov;224:112306. doi: 10.1016/j.jphotobiol.2021.112306. Epub 2021 Sep 10.
Water-filtered infrared A and visible light (wIRA/VIS), shown to reduce chlamydial infections in vitro and in vivo, might represent an innovative therapeutic approach against trachoma, a neglected tropical disease caused by ocular infection with the bacterium C. trachomatis. In this in vivo study, we assessed the impact of wIRA radiation in combination with VIS (wavelength range 595-1400 nm, intensity 2100 W/m) on the retina and cornea in a guinea pig animal model of inclusion conjunctivitis. We investigated the effects 19 days after wIRA/VIS irradiation by comparing a single and double wIRA/VIS treatment with a sham control. By immunolabeling and western blot analyses of critical heat- and stress-responsive proteins, we could not detect wIRA/VIS-induced changes in their expression pattern. Also, immunolabeling of specific retinal marker proteins revealed no changes in their expression pattern caused by the treatment. Our preclinical study suggests wIRA/VIS as a promising and safe therapeutic tool to treat ocular chlamydial infections.
水过滤红外 A 和可见光（wIRA/VIS）已被证明可减少体外和体内的衣原体感染，可能代表了一种针对沙眼的创新治疗方法，沙眼是一种由眼部感染衣原体引起的被忽视的热带病。在这项体内研究中，我们评估了 wIRA 辐射与可见光（波长范围 595-1400nm，强度 2100W/m）联合对包含性结膜炎豚鼠动物模型的视网膜和角膜的影响。我们通过比较单次和双次 wIRA/VIS 治疗与假对照，在 wIRA/VIS 照射 19 天后研究了其影响。通过对关键热应激响应蛋白的免疫标记和 Western blot 分析，我们未发现 wIRA/VIS 诱导其表达模式发生变化。此外，特定视网膜标记蛋白的免疫标记也未显示出治疗引起的其表达模式的变化。我们的临床前研究表明，wIRA/VIS 是一种有前途且安全的治疗眼部衣原体感染的工具。
