Frohns A., Frohns F.
Department of Plant Membrane Biophysics, Technical University of Darmstadt, Darmstadt, Germany
wIRA has been shown to reduce chlamydial infections in vitro and in vivo and might therefore offer an innovative therapeutic approach for fighting trachoma. However, since the eye is a highly temperature- and radiation-sensitive organ, a safety assessment of the ocular structures affected by wIRA treatment is required to establish wIRA as a potentially successful treatment option for clinical application. A prerequisite for this is to demonstrate that wIRA does not have adverse side-effects such as inducing a non-physiological temperature increase which causes cell stress and damage to ocular tissues and which, in turn, is ultimately associated with impaired vision. Likewise, the potential negative impact of non-thermal photochemical effects of wIRA irradiation needs to be investigated. Data from our ex vivo studies in pig and mouse models, as well as in vivo data in a guinea pig model, provide good evidence for the safe use of wIRA to treat chlamydial infections. These studies have excluded a non-physiological temperature rise as well as the activation of heat and stress-induced proteins after wIRA irradiation with therapy-relevant irradiances. Nevertheless, additional detailed in vitro and in vivo studies are needed to further advance the clinical use of wIRA.
弱强度红外A（wIRA）已被证明在体外和体内均可减少衣原体感染，因此可能为治疗沙眼提供一种创新的治疗方法。然而，由于眼睛是一个对温度和辐射高度敏感的器官，需要对受wIRA治疗影响的眼部结构进行安全性评估，以便将wIRA确立为一种潜在成功的临床应用治疗选择。对此的一个先决条件是证明wIRA不会产生不良副作用，例如引起非生理性温度升高，从而导致细胞应激和眼部组织损伤，进而最终导致视力受损。同样，需要研究wIRA照射的非热光化学效应的潜在负面影响。我们在猪和小鼠模型中的离体研究数据，以及豚鼠模型中的体内数据，为安全使用wIRA治疗衣原体感染提供了充分证据。这些研究排除了在具有治疗相关辐照度的wIRA照射后出现非生理性温度升高以及热和应激诱导蛋白的激活。尽管如此，仍需要进行更多详细的体外和体内研究，以进一步推进wIRA的临床应用。
