Stemberger H, Rauhs R, Thetter O, Tamer A, Mayr W R, Kux M
Wien Klin Wochenschr. 1978 Mar 17;90(6):197-200.
26 pig kidneys were perfused in a "Gambro" kidney perfusion machine. The perfusates used were unmodified canine plasma and serum, as well as complement-free plasma, gamma-globulin solutions obtained from dogs immunized against pig leucocytes (ALG) and 2-mercapto-ethanol treated ALG (2 ME-ALG). The typical pig-to-dog xenograft rejection was reproduced by ex vivo perfusion with native dog plasma and serum. Using complement-free dog plasma, ALG and 2 ME-ALG pig kidneys could be perfused for several hours without significant increase in perfusion resistance. 22 pig kidneys, pretreated by perfusion for 2 hours with complement-free dog plasma, ALG and 2 ME-ALG, were transplanted on to the vessels of the neck of recipient dogs. Grafts pretreated with decomplemented dog plasma and 2 ME-ALG were rejected in the usual fashion within 5 to 20 min. Pretreatment with ALG resulted in a prolongation of graft function for 80 to 100 min. It is suggested that ALG exerted a transient blocking effect on the cytotoxic action of recipient IgM resembling passive enhancement; this phenomenon may be limited to sites with low antigen density, corresponding to in vitro findings of passive enhancement on cells with low surface antigen density.
26个猪肾在一台“甘布罗”肾灌注机中进行灌注。所使用的灌注液为未修饰的犬血浆和血清，以及无补体血浆、从经猪白细胞免疫的犬获得的γ球蛋白溶液（抗淋巴细胞球蛋白，ALG）和经2-巯基乙醇处理的ALG（2ME-ALG）。用天然犬血浆和血清进行体外灌注可重现典型的猪对犬异种移植排斥反应。使用无补体犬血浆、ALG和2ME-ALG时，猪肾可灌注数小时而灌注阻力无显著增加。22个经无补体犬血浆、ALG和2ME-ALG灌注预处理2小时的猪肾，被移植到受体犬颈部的血管上。经去补体犬血浆和2ME-ALG预处理的移植物在5至20分钟内以通常方式被排斥。用ALG预处理可使移植物功能延长80至100分钟。提示ALG对受体IgM的细胞毒性作用发挥了类似被动增强的短暂阻断作用；这种现象可能仅限于抗原密度低的部位，这与体外对表面抗原密度低的细胞的被动增强研究结果相符。
