Henry M L, Han L K, Davies E A, Sedmak D D, Ferguson R M
Department of Surgery, Ohio State University College of Medicine, Columbus 43210.
Surgery. 1994 Mar;115(3):355-61.
The lack of human organ donors has prompted a renewed interest in xenotransplantation. Xenoantibody is believed to be an initiator of a complex cascade of events ultimately ending in rapid xenograft destruction.
Cell-free plasma was obtained via plasmapheresis of the recipient canine. This plasma was then perfused in an ex vivo fashion through either the donor (pig) spleen or liver, allowing for specific antidonor antibody deposition in the "screening" organ, and is then returned to the animal. A porcine kidney is then transplanted to the dog, and the outcome is observed.
We have used specific antibody depletion to prolong xenotransplant survival. In the untreated pig-to-dog combination, the transplanted pig kidney is destroyed by the dog in 13 minutes (mean). Adsorption using either the donor spleen or liver resulted in an increase of 3.4 hours and 7.8 hours of graft survival, respectively. The histologic picture of rejected kidneys after adsorption shows a modified form of rejection.
In this pig-to-dog xenograft combination, xenoantibody adsorption allows for prolonged graft survival as compared with control animals. Subsequent xenograft destruction occurs but in a modified manner.
人类器官供体的短缺引发了对异种移植的新兴趣。异种抗体被认为是一系列复杂事件的启动因素，最终导致异种移植物迅速被破坏。
通过对受体犬进行血浆置换获得无细胞血浆。然后将该血浆以离体方式灌注到供体（猪）的脾脏或肝脏中，使特异性抗供体抗体沉积在“筛选”器官中，随后再回输给动物。接着将猪肾移植到犬体内，并观察结果。
我们使用特异性抗体清除来延长异种移植的存活时间。在未经处理的猪 - 犬组合中，移植的猪肾在13分钟（平均）内被犬破坏。使用供体脾脏或肝脏进行吸附分别使移植物存活时间增加了3.4小时和7.8小时。吸附后被排斥肾脏的组织学表现显示为一种改良形式的排斥反应。
在这种猪 - 犬异种移植组合中，与对照动物相比，异种抗体吸附可延长移植物存活时间。随后会发生异种移植物破坏，但方式有所改变。
