Baillou Ambre, Kasal-Hoc Nathalie, Barc Céline, Cognié Juliette, Pinard Anne, Pezant Jérémy, Schulthess Julie, Peltier-Pain Pauline, Lacroix-Lamandé Sonia, Laurent Fabrice
UMR1282 Infectiologie et Santé Publique, INRAE Centre Val de Loire, Université François Rabelais de Tours, 37380 Nouzilly, France.
Phileo by Lesaffre, 137 rue Gabriel Péri, 59700 Marcq-en-Barœul, France.
Vet Sci. 2021 Aug 24;8(9):170. doi: 10.3390/vetsci8090170.
Enteric infectious diseases are not all well controlled, which leads to animal suffering and sometimes death in the most severe cases, in addition to economic losses for farmers. Typical symptoms of enteric infections include watery diarrhea, stomach cramps or pain, dehydration, nausea, vomiting, fever and weight loss. Evaluation of new control methods against enteric infections requires the use of many animals. We aimed to develop a new method for an initial in vivo screen of promising compounds against neonatal diseases such as cryptosporidiosis while limiting experimental animal use. We therefore adapted an in vivo method of multiple consecutive but independent intestinal loops to newborn lambs delivered by cesarean section, in which endotoxin responsiveness is retained. This new method allowed for the screening of natural yeast fractions for their ability to stimulate immune responses and to limit early development. This model may also be used to investigate host-pathogen interactions and immune responses in a neonatal controlled environment.
肠道传染病并未得到完全控制，这不仅给农民带来经济损失，还会导致动物遭受痛苦，在最严重的情况下甚至会死亡。肠道感染的典型症状包括水样腹泻、胃痉挛或疼痛、脱水、恶心、呕吐、发热和体重减轻。评估针对肠道感染的新控制方法需要使用大量动物。我们旨在开发一种新方法，用于初步体内筛选有前景的化合物，以对抗隐孢子虫病等新生儿疾病，同时限制实验动物的使用。因此，我们将一种体内多个连续但独立肠袢的方法应用于剖宫产出生的新生羔羊，这些羔羊保留了内毒素反应性。这种新方法能够筛选天然酵母组分刺激免疫反应和限制早期发育的能力。该模型还可用于研究新生儿可控环境中的宿主-病原体相互作用和免疫反应。
