Clinical Center of Montenegro, Center for Abdominal Surgery, Podgorica, Montenegro.
J BUON. 2021 Jul-Aug;26(4):1466-1478.
The purpose of our work was to investigate the association between proliferative index [proIDX] and expression index p53 (p53IDX) with the clinical and pathological characteristics of gastric adenocarcinoma.
The biopsy material of 90 patients operated on for gastric cancer was routinely processed in paraffin and archived. After the histopathological report was made, two study groups were formed, the first group (n=45) comprised biopsies with intestinal carcinoma and the second (n=45) biopsies of diffuse gastric cancer. In both cases, the control group consisted of biopsies of surrounding non-tumor tissue The routine Hematoxylin-Eosin and immunohistochemical ABC method with anti-Ki67 and anti-p53 antibodies was applied at sections 3-5 μm thick. The expression of Ki67 and p53 was quantified stereometrically. For statistical analysis SPSS (19.0) was used.
Significantly higher Ki67 expression was found in both types of adenocarcinoma compared to the control group, as well as significant association of proIDX with most of testing parameters. Expression of p53 was significantly higher in the intestinal type compared to the diffuse type and the control group and was significantly associated with age and histological grade. Diffuse type particulary showed, significant association of p53IDX with most of the histological parameters tested.
Our results point a highly significant correlation of the Ki67 and p53 expression with indicators of gastric adenocarcinoma progression, which may help to identify patients with an aggressive gastric adenocarcinoma phenotype.
我们的工作目的是研究增殖指数[proIDX]和 p53 表达指数（p53IDX）与胃腺癌临床病理特征的关系。
对 90 例接受胃癌手术的患者活检材料进行常规石蜡包埋和存档。在获得组织病理学报告后，我们将患者分为两组，第一组（n=45）包括肠型腺癌活检，第二组（n=45）包括弥漫型胃癌活检。在这两种情况下，对照组均由周围非肿瘤组织的活检组成。我们应用常规苏木精-伊红和免疫组织化学 ABC 法，使用抗 Ki67 和抗 p53 抗体，对 3-5μm 厚的切片进行染色。使用立体计量学方法对 Ki67 和 p53 的表达进行定量。统计分析采用 SPSS（19.0）。
与对照组相比，两种类型的腺癌中 Ki67 的表达均显著升高，且 proIDX 与大多数检测参数均显著相关。肠型腺癌中 p53 的表达显著高于弥漫型和对照组，且与年龄和组织学分级显著相关。弥漫型特别表现出，p53IDX 与大多数测试的组织学参数显著相关。
我们的研究结果表明，Ki67 和 p53 的表达与胃腺癌进展的指标高度相关，这可能有助于识别具有侵袭性胃腺癌表型的患者。
