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定位于药物代谢细胞色素P - 450c基因上游远处的调控DNA元件。

Regulatory DNA elements localized remotely upstream from the drug-metabolizing cytochrome P-450c gene.

作者信息

Fujisawa-Sehara A, Sogawa K, Nishi C, Fujii-Kuriyama Y

出版信息

Nucleic Acids Res. 1986 Feb 11;14(3):1465-77. doi: 10.1093/nar/14.3.1465.

DOI:10.1093/nar/14.3.1465
PMID:3456557
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC339517/
Abstract

We have investigated regulatory DNA elements in the expression of the drug metabolizing P-450c gene of rats. After combining the 5' flanking and upstream untranslated regions of the isolated P-450c gene with structural gene for chloramphenicol acetyltransferase (CAT), the fusion genes were transfected into cultured cells (Hepa-1 and L cells) for the assay of transient expression of CAT activity. CAT activity was expressed inducibly in response to 3-methylcholanthrene only in Hepa-1 cells. At least three regions containing regulatory DNA elements were indentified; one, which is present in the sequence from -44b to -0.2kb immediately upstream of the TATA box, functions in the basal level of transcription, and the other two which were located in the sequence from -0.8kb to -1.0kb and from -1.0kb to -6.3kb, enhance in combination, transcription in response to inducers in a manner independent of their orientation.

摘要

我们研究了大鼠药物代谢P-450c基因表达中的调控DNA元件。将分离出的P-450c基因的5'侧翼和上游非翻译区与氯霉素乙酰转移酶（CAT）的结构基因相结合后，将融合基因转染到培养细胞（Hepa-1和L细胞）中，用于检测CAT活性的瞬时表达。仅在Hepa-1细胞中，CAT活性可被3-甲基胆蒽诱导表达。至少鉴定出三个含有调控DNA元件的区域；一个位于TATA框上游紧挨着的-44b至-0.2kb序列中，在基础转录水平起作用，另外两个位于-0.8kb至-1.0kb以及-1.0kb至-6.3kb序列中，它们共同作用可增强对诱导剂的转录反应，且这种增强作用与它们的方向无关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b8e/339517/2ecfc7abcafa/nar00272-0339-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b8e/339517/146cf4521c92/nar00272-0337-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b8e/339517/2ecfc7abcafa/nar00272-0339-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b8e/339517/146cf4521c92/nar00272-0337-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b8e/339517/2ecfc7abcafa/nar00272-0339-a.jpg

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