Hynes N, van Ooyen A J, Kennedy N, Herrlich P, Ponta H, Groner B
Proc Natl Acad Sci U S A. 1983 Jun;80(12):3637-41. doi: 10.1073/pnas.80.12.3637.
After transfection of mouse mammary tumor virus (MMTV) proviral DNA into cultured cells, the DNA is transcribed in a glucocorticoid-sensitive fashion. The large terminal repeat (LTR) region of MMTV is 1,328 nucleotides long and contains the regulatory information necessary for the hormonal response. We have constructed a MMTV LTR-thymidine kinase (tk) chimeric gene and have tested the biological activity of molecules containing various deletions in the LTR after transformation of LTK- APRT- mouse cells. In the TK+ transformants, both a LTR- tk chimeric RNA and an authentic tk RNA are correctly initiated and transcribed. The synthesis of the chimeric RNA as well as that of the tk RNA is hormonally regulated. A plasmid containing 202 nucleotides of LTR DNA 5' to the RNA initiation site is fully sensitive to glucocorticoids; 50 nucleotides still cause a residual inducibility.
将小鼠乳腺肿瘤病毒（MMTV）前病毒DNA转染到培养细胞后，该DNA以糖皮质激素敏感的方式进行转录。MMTV的大末端重复序列（LTR）区域长1328个核苷酸，包含激素反应所需的调控信息。我们构建了一个MMTV LTR-胸苷激酶（tk）嵌合基因，并在LTK- APRT-小鼠细胞转化后，测试了含有LTR中各种缺失的分子的生物学活性。在TK+转化体中，LTR- tk嵌合RNA和真实的tk RNA均能正确起始和转录。嵌合RNA以及tk RNA的合成受激素调节。一个在RNA起始位点5'端含有202个核苷酸LTR DNA的质粒对糖皮质激素完全敏感；50个核苷酸仍会引起残余诱导性。
