The Role of Spectrophotometric Standards in the Clinical Chemistry Laboratory.

It is obvious that erroneous data reported to a physician may adversely affect patient welfare. Currently, acceptable limits of accuracy and precision are poorly defined. It should be recognized, however, that the spectrophotometric measurement step in an appropriate analytical procedure is critical and inapparent error may occur. Spectrophotometric measurements, both manual and automated, are extensively used in the clinical chemistry laboratory. At least 1,000,000 such measurements per day on rather diverse equipment are made in this country; yet, few standards exist. Results of intra-lab surveys suggests that performance could be improved. The various ways in which spectrophotometry is used will be illustrated and a discussion of possible errors resulting from nonstandardized instrumentation will follow. There is pressing need for well defined and easily usable standards for wavelength, photometric accuracy, photometric linearity, stray light, as well as NBS specifications for optical cuvettes.