Park Joon-Heum, Jung Sunyo
School of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook National University, Daegu 41566, Korea.
J Pestic Sci. 2021 Aug 20;46(3):258-266. doi: 10.1584/jpestics.D21-021.
We examined the effect of () expression on the regulation of porphyrin biosynthesis and resistance to norflurazon (NF)-induced photobleaching in transgenic rice. In response to NF, transgenic lines F4 and F7 showed lesser declines in chlorophyll, carotenoid, / , ϕ, and light-harvesting chlorophyll (Lhc) /-binding proteins as compared to wild-type (WT) plants, resulting in the alleviation of NF-induced photobleaching. During photobleaching, levels of heme, protoporphyrin IX (Proto IX), Mg-Proto IX (monomethylester), and protochlorophyllide decreased in WT and transgenic plants, with lesser decreases in transgenic plants. Most porphyrin biosynthetic genes were greatly downregulated in WT and transgenic plants following NF treatment, with higher transcript levels in transgenic plants. The expression of in transgenic rice may play a protective role in mitigating NF-induced photobleaching by maintaining levels of heme, chlorophyll intermediates, and Lhc proteins. This finding will contribute to understanding the resistance mechanism of NF-resistant crops and establishing a new strategy for weed control.
我们研究了（）表达对转基因水稻中卟啉生物合成调控以及对氟草敏（NF）诱导的光漂白抗性的影响。响应NF时，与野生型（WT）植株相比，转基因株系F4和F7在叶绿素、类胡萝卜素、/、ϕ以及捕光叶绿素（Lhc）/-结合蛋白方面的下降幅度较小，从而减轻了NF诱导的光漂白。在光漂白过程中，WT和转基因植株中的血红素、原卟啉IX（Proto IX）、Mg-原卟啉IX（单甲酯）和原叶绿素酸酯水平均下降，转基因植株中的下降幅度较小。在NF处理后，大多数卟啉生物合成基因在WT和转基因植株中均大幅下调，而转基因植株中的转录水平较高。转基因水稻中的表达可能通过维持血红素、叶绿素中间体和Lhc蛋白的水平，在减轻NF诱导的光漂白方面发挥保护作用。这一发现将有助于理解耐NF作物的抗性机制，并建立一种新的杂草控制策略。
