Wang Qiye, Zeng Yutong, Zeng Xianglin, Wang Xin, Wang Yancan, Dai Chunpeng, Li Jianzhong, Huang Pengfei, Huang Jing, Hussain Tarique, Zhu Mingzhi, Yang Huansheng
Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Laboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha, China.
Hubei Zhiqinghe Agriculture and Animal Husbandry Co., Ltd., Yichang, China.
Front Microbiol. 2021 Sep 10;12:695445. doi: 10.3389/fmicb.2021.695445. eCollection 2021.
This study investigated rumen fermentation and histological and microbial diversity in male Hu lamb fed diets with different metabolizable energy (ME) levels (MEA, 9.17 MJ/kg, MEB, 10.00 MJ/kg, and MEC, 10.82 MJ/kg). Thirty-six male Hu lambs were randomly allotted to three treatments, and the feeding trial lasted for 67 days. Rumen fermentation results suggest that the iso-valerate had a significant effect on dietary energy level. The papillary height (PH) of rumen was the highest in the MEB group, the crypt depth (CD) was significantly increased in the duodenum and jejunum, and the villus height (VH)-to-CD ratio (VH/CD) was significantly decreased in the duodenum by increasing dietary energy levels; the VH, villus width (VW), and VH/CD also had significant differences in the ileum. 16S rRNA sequencing results showed that the operational taxonomic units (OTUs) number, the ACE, and Chao1 indices were linearly decreased by increasing dietary energy level; 24 phyla including 124 genera were identified, and the relative abundance of and linearly decreased by increasing the dietary energy level. Compared to MEA and MEB groups, the relative abundance of and was significantly increased in the MEC group at the genus level. The relative abundance of the carbohydrate metabolism pathway predicted by Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) was linearly increased by increasing the dietary energy levels. Three metabolic pathways identified in Kyoto Encyclopedia of Genes and Genomes (KEGG) level 3 were significantly influenced as the dietary energy level increased. In summary, these results demonstrated that the dietary energy levels affected the rumen fermentation parameters, morphological structures of the gastrointestinal tract (GIT), and the composition and function of rumen microflora in male Hu sheep.
本研究调查了饲喂不同代谢能（ME）水平（MEA，9.17兆焦/千克；MEB，10.00兆焦/千克；MEC，10.82兆焦/千克）日粮的雄性湖羊羔羊的瘤胃发酵、组织学及微生物多样性。36只雄性湖羊羔羊被随机分配到三种处理组，饲养试验持续67天。瘤胃发酵结果表明，异戊酸对日粮能量水平有显著影响。瘤胃乳头高度（PH）在MEB组最高，十二指肠和空肠的隐窝深度（CD）显著增加，十二指肠绒毛高度（VH）与CD之比（VH/CD）随日粮能量水平升高而显著降低；回肠的VH、绒毛宽度（VW）和VH/CD也存在显著差异。16S rRNA测序结果显示，可操作分类单元（OTU）数量、ACE和Chao1指数随日粮能量水平升高呈线性下降；鉴定出包括124个属的24个门， 和 的相对丰度随日粮能量水平升高呈线性下降。与MEA组和MEB组相比，MEC组在属水平上 和 的相对丰度显著增加。通过未观察状态重建群落系统发育研究（PICRUSt）预测的碳水化合物代谢途径的相对丰度随日粮能量水平升高呈线性增加。京都基因与基因组百科全书（KEGG）第3级鉴定的三条代谢途径随日粮能量水平升高受到显著影响。总之，这些结果表明日粮能量水平影响雄性湖羊的瘤胃发酵参数、胃肠道（GIT）形态结构以及瘤胃微生物区系的组成和功能。
