Laboratory of Animal Nutrition and Human Health, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, College of Life Science, Hunan Normal University, Changsha, Hunan, China.
Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, China.
J Anim Sci. 2020 Mar 1;98(3). doi: 10.1093/jas/skaa046.
Weaning stresses often induce markedly structural and functional changes in the small intestine. However, little data are available on the changes of the morphology, function, and health in the cecum of piglets post-weaning. This study was conducted to measure the effects of weaning on the cecum in piglets. Forty piglets were weaned at 21 d and samples were collected at different time points (days 0, 1, 3, 7, and 14) post-weaning. The crypt depth, crypt width, and total epithelial cell numbers increased on days 7 and 14, compared with days 0, 1, and 3 (P < 0.001). The value of Ki67/total epithelial cells was highest on day 0 compared with all other days (P < 0.05). Besides, a higher concentration of malondialdehyde was observed on day 3 (P < 0.001). The activity of CuZn-superoxide dismutase (P < 0.05) enhanced from day 0 to 1 and the activity of catalase increased from day 1 to 3 (P < 0.001). In addition, days 3, 7, and 14 had greater acetic, propanoic, and butyric acid contents than on day 1 (P < 0.001). The pH and monocarboxylate transporter 1 (MCT1) expression increased from day 0 to 1 and from day 3 to 7 and decreased between days 1 and 3 (P < 0.001). The mRNA expression of solute carrier family 9 member A3 (SLC9A3) decreased on day 1 compared to all other postweaning days (P < 0.05). The abundance of toll-like receptor 4 (TLR4) and interferon-γ (IFN-γ) mRNA expression increased (P < 0.05) during the first 24 h after weaning. The concentration of lipopolysaccharide increased from day 3 to 7, then decreased on day 14 (P < 0.05). The mRNA expression of tumor necrosis factor-α (TNF-α) enhanced from day 7 to 14 (P < 0.05). The abundance of phosphorylated mammalian target of rapamycin protein was lower on day 14 than day 0 (P < 0.05). Taken together, these results show that weaning in piglets influences intestinal morphology, function, and health in the cecum.
断奶应激通常会导致小肠明显的结构和功能变化。然而，关于断奶后仔猪盲肠的形态、功能和健康变化的数据很少。本研究旨在测量断奶对仔猪盲肠的影响。40 头仔猪于 21 日龄断奶，并在断奶后不同时间点（0、1、3、7 和 14 天）采集样本。与 0、1 和 3 天相比，断奶后第 7 和 14 天隐窝深度、隐窝宽度和总上皮细胞数量增加（P<0.001）。第 0 天 Ki67/总上皮细胞的值高于其他所有天数（P<0.05）。此外，第 3 天丙二醛浓度较高（P<0.001）。CuZn-超氧化物歧化酶的活性从第 0 天到第 1 天增强（P<0.05），过氧化氢酶的活性从第 1 天到第 3 天增加（P<0.001）。此外，第 3、7 和 14 天的乙酸、丙酸和丁酸含量均高于第 1 天（P<0.001）。pH 值和单羧酸转运蛋白 1（MCT1）的表达从第 0 天到第 1 天以及从第 3 天到第 7 天增加，然后在第 1 天和第 3 天之间减少（P<0.001）。与断奶后所有其他天数相比，第 1 天溶质载体家族 9 成员 A3（SLC9A3）的 mRNA 表达降低（P<0.05）。断奶后 24 小时内，Toll 样受体 4（TLR4）和干扰素-γ（IFN-γ）mRNA 表达增加（P<0.05）。脂多糖的浓度从第 3 天到第 7 天增加，然后在第 14 天减少（P<0.05）。肿瘤坏死因子-α（TNF-α）的 mRNA 表达从第 7 天到第 14 天增强（P<0.05）。第 14 天磷酸化哺乳动物雷帕霉素靶蛋白的丰度低于第 0 天（P<0.05）。综上所述，这些结果表明，仔猪断奶会影响盲肠的肠道形态、功能和健康。
