State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, China.
Front Immunol. 2021 Sep 9;12:711329. doi: 10.3389/fimmu.2021.711329. eCollection 2021.
Dendritic cells (DCs) play essential roles in innate and adaptive immunity and show high heterogeneity and intricate ontogeny. Advances in high-throughput sequencing technologies, particularly single-cell RNA sequencing (scRNA-seq), have improved the understanding of DC subsets. In this review, we discuss in detail the remarkable perspectives in DC reclassification and ontogeny as revealed by scRNA-seq. Moreover, the heterogeneity and multifunction of DCs during diseases as determined by scRNA-seq are described. Finally, we provide insights into the challenges and future trends in scRNA-seq technologies and DC research.
树突状细胞 (DCs) 在先天免疫和适应性免疫中发挥着重要作用，具有高度异质性和复杂的发生。高通量测序技术的进步，特别是单细胞 RNA 测序 (scRNA-seq)，提高了对 DC 亚群的认识。在这篇综述中，我们详细讨论了 scRNA-seq 揭示的 DC 重新分类和发生的显著观点。此外，还描述了 scRNA-seq 确定的 DC 在疾病中的异质性和多功能性。最后，我们对 scRNA-seq 技术和 DC 研究中的挑战和未来趋势提供了一些见解。
