Dong Xiaonan, Liu Chaorui, Wang Yuqi, Dong Qing, Gai Yingping, Ji Xianling
College of Forestry, Shandong Agricultural University, Tai'an, China.
State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, China.
Front Plant Sci. 2021 Sep 8;12:687364. doi: 10.3389/fpls.2021.687364. eCollection 2021.
To understand the mechanism of small non-coding RNAs (miRNA)-mediated development and ripening of mulberry fruits, three small RNA libraries from mulberry fruits at different development stages were constructed, and 159 conserved miRNAs as well as 86 novel miRNAs were successfully identified. Among the miRNAs identified, there were 90 miRNAs which showed differential expression patterns at different stages of fruit development and ripening. The target genes of these differential expressed (DE) miRNAs were involved in growth and development, transcription and regulation of transcription, metabolic processes, and etc. Interestingly, it was found that the expression level of mul-miR477 was increased with fruit ripening, and it can target the antisense lncRNA () of the ATP binding cassette (ABC) transporter B 19 gene (). Our results showed that mul-miR477 can repress the expression of and increase the expression of , and it acted as a positive regulator participating anthocyanin accumulation through the regulatory network of mul-miR477--.
为了解小非编码RNA（miRNA）介导的桑椹果实发育和成熟机制，构建了来自桑椹果实不同发育阶段的三个小RNA文库，并成功鉴定出159个保守miRNA以及86个新的miRNA。在鉴定出的miRNA中，有90个miRNA在果实发育和成熟的不同阶段表现出差异表达模式。这些差异表达（DE）miRNA的靶基因参与生长发育、转录及转录调控、代谢过程等。有趣的是，发现mul-miR477的表达水平随果实成熟而增加，并且它可以靶向ATP结合盒（ABC）转运蛋白B19基因（）的反义lncRNA（）。我们的结果表明，mul-miR477可以抑制的表达并增加的表达，并且它通过mul-miR477--调控网络作为参与花青素积累的正调控因子。
