• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基因突变（）在临床分离株对环丙沙星耐药性中的作用。 你提供的原文中括号里内容缺失，可能会影响对完整意思的理解。

The role of Gene Mutations () in Resistance to Ciprofloxacin in Clinical Isolates of .

作者信息

Arabameri Nasibeh, Heshmatipour Zoheir, Eftekhar Ardebili Shima, Jafari Bidhendi Zeinab

机构信息

Department of Microbiology, Faculty of Science, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran.

出版信息

Iran J Pathol. 2021 Fall;16(4):426-432. doi: 10.30699/IJP.2021.520570.2542. Epub 2021 Jul 6.

DOI:10.30699/IJP.2021.520570.2542
PMID:34567192
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8463757/
Abstract

BACKGROUND & OBJECTIVE: is an opportunistic pathogen and one of the most common causes of nosocomial infections. This bacterium's antibiotic resistance to the common fluoroquinolone antibiotics, especially ciprofloxacin, is due to mutations in the and genes. This study aimed to investigate the effect of the mutation in (, ) on ciprofloxacin resistance in clinical isolates of .

METHODS

A total of 140 clinical samples were collected from hospitals. The samples were identified by standard biochemical tests, and the antibiotic resistance was investigated by the disk diffusion method. DNA was extracted from 30 isolates, and PCR was performed. PCR-sequencing was carried out to assess and mutations in drug-resistant isolates. NCBI-Blast and MEGA7 software was used to analyze the nucleotide sequences.

RESULTS

30 clinical isolates were 80% resistant to ciprofloxacin; meanwhile, in 21 samples, mutations were observed. 87/5% of mutations were related to (Thr83 → Ile), 79/16 % (Ser87 → Leu), and 4/18% (Glu91 → Lys). The antibiotic resistance to ciprofloxacin and mutations in and genes in resistant isolates are significantly related to each other (<0.05).

CONCLUSION

The mutations in the and genes play an essential role in resistance to ciprofloxacin in clinical isolates of .

摘要

背景与目的

[细菌名称]是一种机会致病菌，也是医院感染最常见的病因之一。该细菌对常见氟喹诺酮类抗生素，尤其是环丙沙星的耐药性，是由于[基因名称1]和[基因名称2]基因发生突变所致。本研究旨在调查[细菌名称]临床分离株中[基因名称1]和[基因名称2]的突变对环丙沙星耐药性的影响。

方法

从医院共收集140份临床样本。通过标准生化试验对样本进行鉴定，并采用纸片扩散法调查抗生素耐药性。从30株分离株中提取DNA，并进行聚合酶链反应（PCR）。对耐药分离株进行PCR测序，以评估[基因名称1]和[基因名称2]的突变情况。使用美国国立生物技术信息中心（NCBI）的Blast和MEGA7软件分析核苷酸序列。

结果

30株临床分离株对环丙沙星的耐药率为80%；同时，在21份样本中观察到突变。87.5%的突变与[基因名称1]（苏氨酸83→异亮氨酸）有关，79.16%与[基因名称2]（丝氨酸87→亮氨酸）有关，4.18%（谷氨酸91→赖氨酸）。耐药分离株对环丙沙星的耐药性与[基因名称1]和[基因名称2]基因的突变之间存在显著相关性（P<0.05）。

结论

[基因名称1]和[基因名称2]基因的突变在[细菌名称]临床分离株对环丙沙星的耐药性中起重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e9/8463757/66874be05924/ijp-16-426-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e9/8463757/8d0fb7c71960/ijp-16-426-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e9/8463757/8da1768d5150/ijp-16-426-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e9/8463757/ebc1f765dd86/ijp-16-426-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e9/8463757/66874be05924/ijp-16-426-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e9/8463757/8d0fb7c71960/ijp-16-426-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e9/8463757/8da1768d5150/ijp-16-426-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e9/8463757/ebc1f765dd86/ijp-16-426-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e9/8463757/66874be05924/ijp-16-426-g004.jpg

相似文献

1
The role of Gene Mutations () in Resistance to Ciprofloxacin in Clinical Isolates of .基因突变（）在临床分离株对环丙沙星耐药性中的作用。 你提供的原文中括号里内容缺失，可能会影响对完整意思的理解。
Iran J Pathol. 2021 Fall;16(4):426-432. doi: 10.30699/IJP.2021.520570.2542. Epub 2021 Jul 6.
2
Characterization of and mutations in ciprofloxacin-resistant isolates from Tehran hospitals in Iran.伊朗德黑兰医院耐环丙沙星分离株中及突变的特征分析。
Iran J Microbiol. 2018 Aug;10(4):242-249.
3
Mutations in the , , and genes provide functional insights into the fluoroquinolone-resistant isolated in Vietnam.在越南分离出的耐氟喹诺酮菌株中，对、和基因的突变进行研究能提供功能方面的见解。 （你提供的原文中基因名称处有缺失内容）
Infect Drug Resist. 2018 Feb 28;11:275-282. doi: 10.2147/IDR.S147581. eCollection 2018.
4
[Susceptibility of 570 Pseudomonas aeruginosa strains to 11 antimicrobial agents and the mechanism of its resistance to fluoroquinolones].[570株铜绿假单胞菌对11种抗菌药物的敏感性及其对氟喹诺酮类耐药机制]
Zhonghua Yi Xue Za Zhi. 2003 Mar 10;83(5):403-7.
5
The role of gyrA and parC mutations in fluoroquinolones-resistant Pseudomonas aeruginosa isolates from Iran.gyrA和parC突变在来自伊朗的耐氟喹诺酮铜绿假单胞菌分离株中的作用。
Braz J Microbiol. 2016 Oct-Dec;47(4):925-930. doi: 10.1016/j.bjm.2016.07.016. Epub 2016 Jul 26.
6
Quinolone-resistant clinical strains of isolated from University Hospital in Tunisia.从突尼斯大学医院分离出的耐喹诺酮临床菌株。
3 Biotech. 2018 Jan;8(1):1. doi: 10.1007/s13205-017-1019-8. Epub 2017 Nov 13.
7
Antimicrobial resistance and novel mutations detected in the gyrA and parC genes of Pseudomonas aeruginosa strains isolated from companion dogs.从伴侣犬分离的铜绿假单胞菌中 gyrA 和 parC 基因的耐药性和新型突变检测。
BMC Vet Res. 2020 Apr 15;16(1):111. doi: 10.1186/s12917-020-02328-0.
8
Association between mutations in gyrA and parC genes of Acinetobacter baumannii clinical isolates and ciprofloxacin resistance.鲍曼不动杆菌临床分离株gyrA和parC基因突变与环丙沙星耐药性之间的关联
Iran J Basic Med Sci. 2015 Jun;18(6):623-6.
9
Alterations in the GyrA and GyrB subunits of topoisomerase II and the ParC and ParE subunits of topoisomerase IV in ciprofloxacin-resistant clinical isolates of Pseudomonas aeruginosa.铜绿假单胞菌环丙沙星耐药临床分离株中拓扑异构酶II的GyrA和GyrB亚基以及拓扑异构酶IV的ParC和ParE亚基的改变。
Int J Antimicrob Agents. 2005 Apr;25(4):290-5. doi: 10.1016/j.ijantimicag.2004.11.012.
10
Gene-Gene Interactions Dictate Ciprofloxacin Resistance in Pseudomonas aeruginosa and Facilitate Prediction of Resistance Phenotype from Genome Sequence Data.基因-基因相互作用决定铜绿假单胞菌对环丙沙星的耐药性，并有助于从基因组序列数据预测耐药表型。
Antimicrob Agents Chemother. 2021 Jun 17;65(7):e0269620. doi: 10.1128/AAC.02696-20.

引用本文的文献

1
Pseudomonas aeruginosa in dental unit waterlines: an emerging threat of virulence genes in healthcare-associated infections.牙科设备水路中的铜绿假单胞菌：医疗保健相关感染中毒力基因的新威胁。
BMC Oral Health. 2025 Aug 11;25(1):1313. doi: 10.1186/s12903-025-06668-x.
2
Comprehensive analysis of extensive drug-resistant Typhi in Gujarat region, India: genomic findings and prospective alternative therapy.印度古吉拉特邦地区广泛耐药伤寒杆菌的综合分析：基因组研究结果及潜在替代疗法
Microbiol Spectr. 2025 Jul;13(7):e0254024. doi: 10.1128/spectrum.02540-24. Epub 2025 May 27.
3
Unraveling the Resistome, Virulome, and Pathogenicity of O157:H7 From Cattle Feces.

本文引用的文献

1
Prevalence and fluoroquinolone resistance of pseudomonas aeruginosa in a hospital of South China.中国南方某医院铜绿假单胞菌的患病率及氟喹诺酮耐药性
Int J Clin Exp Med. 2015 Jan 15;8(1):1386-90. eCollection 2015.
2
Antimicrobial resistance and molecular typing of pseudomonas aeruginosa isolated from surgical wounds in Lagos, Nigeria.从尼日利亚拉各斯手术伤口分离出的铜绿假单胞菌的抗菌耐药性及分子分型
Acta Med Iran. 2012;50(6):433-8.
3
gyrA and parC mutations in quinolone-resistant clinical isolates of Pseudomonas aeruginosa from Nini Hospital in north Lebanon.
解析牛粪便中O157:H7的耐药基因组、毒力基因组及致病性
Int J Microbiol. 2025 Feb 5;2025:5087461. doi: 10.1155/ijm/5087461. eCollection 2025.
4
Unveiling the nanoworld of antimicrobial resistance: integrating nature and nanotechnology.揭开抗微生物耐药性的纳米世界：融合自然与纳米技术
Front Microbiol. 2025 Jan 9;15:1391345. doi: 10.3389/fmicb.2024.1391345. eCollection 2024.
5
Resistome phylodynamics of multidrug-resistant isolated from diarrheal patients.从腹泻患者中分离出的多重耐药菌的抗性组系统发育动力学
Microbiol Spectr. 2025 Jan 7;13(1):e0163524. doi: 10.1128/spectrum.01635-24. Epub 2024 Nov 29.
6
Evaluation of Antimicrobial Resistancein Clinical Isolates of spp. Obtained from Hospital Patients in Latvia.评估从拉脱维亚医院患者中分离得到的 spp. 临床分离株的耐药性。
Medicina (Kaunas). 2024 May 23;60(6):850. doi: 10.3390/medicina60060850.
7
Biofilm Formation, Pyocyanin Production, and Antibiotic Resistance Profile of Isolates from Wounds.伤口分离株的生物膜形成、绿脓菌素产生及抗生素耐药谱
Int J Microbiol. 2024 Feb 20;2024:1207536. doi: 10.1155/2024/1207536. eCollection 2024.
8
The First Saudi Report of Novel and Common Mutations in the and Genes Among Spp. Clinical Isolates Recovered from Taif Area.从塔伊夫地区分离出的临床分离株中，沙特关于 spp. 中 基因和 基因新突变及常见突变的第一份报告 。 你提供的原文似乎存在信息不完整的情况，部分基因名称未明确写出，可能会影响准确理解。
Infect Drug Resist. 2022 Jul 16;15:3801-3814. doi: 10.2147/IDR.S372027. eCollection 2022.
黎巴嫩北部尼尼医院耐喹诺酮类药物铜绿假单胞菌临床分离株中 gyrA 和 parC 突变。
J Infect Chemother. 2013 Feb;19(1):77-81. doi: 10.1007/s10156-012-0455-y. Epub 2012 Jul 21.
4
Resistance of bacterial biofilms to disinfectants: a review.细菌生物膜对消毒剂的抗性：综述。
Biofouling. 2011 Oct;27(9):1017-32. doi: 10.1080/08927014.2011.626899.
5
DNA topoisomerases and their poisoning by anticancer and antibacterial drugs.DNA拓扑异构酶及其被抗癌和抗菌药物抑制的情况。
Chem Biol. 2010 May 28;17(5):421-33. doi: 10.1016/j.chembiol.2010.04.012.
6
Prevalence of ESBLs genes among multidrug-resistant isolates of Pseudomonas aeruginosa isolated from patients in Tehran.在德黑兰的患者中分离的多重耐药铜绿假单胞菌中 ESBLs 基因的流行率。
Microb Drug Resist. 2009 Mar;15(1):37-9. doi: 10.1089/mdr.2009.0880.
7
Bacterial infections in burn patients.烧伤患者的细菌感染
Indian J Med Res. 2008 Apr;127(4):415; author reply 416.
8
Resistance mechanisms of multiresistant Pseudomonas aeruginosa strains from Germany and correlation with hypermutation.德国多重耐药铜绿假单胞菌菌株的耐药机制及其与超突变的相关性
Antimicrob Agents Chemother. 2007 Nov;51(11):4062-70. doi: 10.1128/AAC.00148-07. Epub 2007 Sep 17.
9
Contributions of efflux pumps to high level resistance of Pseudomonas aeruginosa to ciprofloxacin.外排泵对铜绿假单胞菌对环丙沙星高水平耐药性的作用。
Chin Med J (Engl). 2007 Jan 5;120(1):68-70.
10
Alterations in the GyrA and GyrB subunits of topoisomerase II and the ParC and ParE subunits of topoisomerase IV in ciprofloxacin-resistant clinical isolates of Pseudomonas aeruginosa.铜绿假单胞菌环丙沙星耐药临床分离株中拓扑异构酶II的GyrA和GyrB亚基以及拓扑异构酶IV的ParC和ParE亚基的改变。
Int J Antimicrob Agents. 2005 Apr;25(4):290-5. doi: 10.1016/j.ijantimicag.2004.11.012.