Dong Xue, Tong Geng, Song Xuankun, Xiao Xingchen, Yu Yiting
Research & Development Institute of Northwestern Polytechnical University in Shenzhen, Shenzhen, 518057 China.
Ningbo Institute of Northwestern Polytechnical University, Ningbo, 315103 China.
Microsyst Nanoeng. 2021 Sep 1;7:68. doi: 10.1038/s41378-021-00299-2. eCollection 2021.
As one of the most common hyperspectral microscopy (HSM) techniques, line-scanning HSM is currently utilized in many fields. However, its scanning efficiency is still considered to be inadequate since many biological and chemical processes occur too rapidly to be captured. Accordingly, in this work, a digital micromirror device (DMD) based on microelectromechanical systems (MEMS) is utilized to demonstrate a flexible multiline scanning HSM system. To the best of our knowledge, this is the first line-scanning HSM system in which the number of scanning lines can be tuned by simply changing the DMD's parallel scanning units according to diverse applications. This brilliant strategy of effortless adjustability relies only on on-chip scanning methods and totally exploits the benefits of parallelization, aiming to achieve nearly an -time improvement in the detection efficiency and an -time decrease in the scanning time and data volume compared with the single-line method under the same operating conditions. To validate this, we selected a few samples of different spectral wavebands to perform reflection imaging, transmission imaging, and fluorescence imaging with varying numbers of scanning lines. The results show the great potential of our DMD-based HSM system for the rapid development of cellular biology, material analysis, and so on. In addition, its on-chip scanning process eliminates the inherent microscopic architecture, making the whole system compact, lightweight, portable, and not subject to site constraints.
作为最常见的高光谱显微镜（HSM）技术之一，线扫描高光谱显微镜目前在许多领域都有应用。然而，由于许多生物和化学过程发生得太快而无法捕捉，其扫描效率仍被认为不足。因此，在这项工作中，一种基于微机电系统（MEMS）的数字微镜器件（DMD）被用于展示一种灵活的多线扫描高光谱显微镜系统。据我们所知，这是首个线扫描高光谱显微镜系统，在该系统中，可以根据不同应用，通过简单改变DMD的并行扫描单元来调整扫描线的数量。这种出色的轻松可调策略仅依赖于片上扫描方法，并充分利用了并行化的优势，旨在在相同操作条件下，与单线方法相比，实现检测效率近倍提高，扫描时间和数据量近倍减少。为了验证这一点，我们选择了几个不同光谱波段的样本，用不同数量的扫描线进行反射成像、透射成像和荧光成像。结果表明，我们基于DMD的高光谱显微镜系统在细胞生物学、材料分析等快速发展方面具有巨大潜力。此外，其片上扫描过程消除了固有的微观结构，使整个系统紧凑、轻便、便携且不受场地限制。
