Serviço de Imunologia, Complexo Hospitalar Universitário Prof. Edgard Santos, Universidade Federal da Bahia, Salvador, Brazil.
Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais - INCT-DT (CNPq/MCT), Salvador, Brazil.
Front Cell Infect Microbiol. 2021 Sep 10;11:740278. doi: 10.3389/fcimb.2021.740278. eCollection 2021.
Disseminated Leishmaniasis (DL) is an emerging and severe form of infection defined by the presence of 10 and up to more than 1,000 skin lesions. The mechanisms underlying parasite dissemination remain unknown. Genotypic differences among species of have been associated with different clinical forms of disease. The present work compared the function of monocytes obtained from patients with cutaneous leishmaniasis (CL) and DL in response to infection with isolates of both these two clinical forms of disease. Mononuclear cells obtained from DL and CL patients were infected with different isolates, and numbers of infected cells, parasite load, respiratory burst, TLR2 and TLR4 expression and cytokine production were evaluated. DL isolates infected more monocytes, induced greater respiratory burst, and more cytokine production compared to isolates from CL patients regardless of the origin of monocytes (DL or CL). However, greater parasite multiplication and higher TLR2 and TLR4 expression were seen in monocytes from DL patients compared to CL following infection with DL isolates. Our results indicate the participation of both parasite genotype and host factors in the pathogenesis of DL.
播散性利什曼病（DL）是一种新兴的严重感染形式，其特征是存在 10 个及以上、多达 1000 多个皮肤损伤。寄生虫播散的机制尚不清楚。利什曼原虫种间的基因型差异与不同的临床疾病形式有关。本研究比较了来自皮肤利什曼病（CL）和 DL 患者的单核细胞在感染这两种临床形式疾病的 分离株后的功能。将来自 DL 和 CL 患者的单核细胞分别用不同的 分离株感染，并评估感染细胞数量、寄生虫负荷、呼吸爆发、TLR2 和 TLR4 表达和细胞因子产生。与来自 CL 患者的分离株相比，无论单核细胞（DL 或 CL）的来源如何，DL 分离株感染更多的单核细胞，诱导更强的呼吸爆发，并产生更多的细胞因子。然而，在感染 DL 分离株后，来自 DL 患者的单核细胞中观察到更高的寄生虫增殖以及更高的 TLR2 和 TLR4 表达。我们的研究结果表明，寄生虫基因型和宿主因素都参与了 DL 的发病机制。
