Hogg N, Takacs L, Palmer D G, Selvendran Y, Allen C
Eur J Immunol. 1986 Mar;16(3):240-8. doi: 10.1002/eji.1830160306.
A new monoclonal antibody (mAb), named 3.9, is described that is specific for the p150,95 molecule, a member of the LFA-1, CR3, p150,95 family of human leukocyte differentiation antigens. The LFA-1 molecule participates in a variety of T cell interactions and the CR3 molecule is the receptor for the complement component iC3b, but little is known about the p150,95 molecule. Here we show that the expression of p150,95 is confined to myeloid cells. mAb 3.9 reacts variably with neutrophils, more strongly with monocytes and is most strongly expressed on tissue macrophages. Using this mAb and others, we have examined the heterogeneity of tissue macrophages. Cells such as Langerhans' cells, dendritic reticulum cells and osteoclasts failed to react with these mAb and thus, probably do not belong to the mononuclear phagocyte lineage. Using a new double-labeling technique, we investigated lymphoid tissue for dendritic cells bearing class II molecules which might function in interactions with T cells. In T cell areas macrophages expressing class II markers were seen but there was no evidence for other types of dendritic or interdigitating cells which expressed class II molecules but not macrophage epitopes. The conclusion from this survey was that the most prominent cell with dendritic morphology found in the T cell areas of lymphoid tissue was a macrophage.
本文描述了一种名为3.9的新型单克隆抗体（mAb），它对p150,95分子具有特异性，p150,95分子是人类白细胞分化抗原LFA-1、CR3、p150,95家族的成员。LFA-1分子参与多种T细胞相互作用，CR3分子是补体成分iC3b的受体，但对p150,95分子了解甚少。在这里，我们表明p150,95的表达仅限于髓样细胞。单克隆抗体3.9与中性粒细胞反应不一，与单核细胞反应更强，在组织巨噬细胞上表达最强。使用这种单克隆抗体和其他抗体，我们研究了组织巨噬细胞的异质性。诸如朗格汉斯细胞、树突状网状细胞和破骨细胞等细胞未能与这些单克隆抗体发生反应，因此可能不属于单核吞噬细胞谱系。使用一种新的双标记技术，我们在淋巴组织中研究了带有可能在与T细胞相互作用中发挥作用的II类分子的树突状细胞。在T细胞区域可见表达II类标记的巨噬细胞，但没有证据表明存在其他类型的表达II类分子但不表达巨噬细胞表位的树突状或交错突细胞。这项研究的结论是，在淋巴组织的T细胞区域发现的具有树突状形态的最突出细胞是巨噬细胞。
