Laboratory of Aquatic Animal Medicine, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea.
Microb Drug Resist. 2022 Feb;28(2):255-265. doi: 10.1089/mdr.2020.0618. Epub 2021 Sep 27.
is a significant cause of infection in both marine animals and humans. It has been reported frequently in seafood-borne infections worldwide. This study was conducted to determine the potential health impact of the isolated from marine food fish cultured in Korea concerning their virulence and antimicrobial resistance. A total of 49 samples were isolated by biochemical tests and multiplex PCR. Phenotypic detection of virulence factors resulted DNase activity (81.63%), hemolysis ( = 75.51% and = 12.25), gelatinase activity (71.43%), protease production (71.43%), phospholipase activity (65.31%), and lipase production (34.69%). Virulence genes, including , , , , , and , were detected in 57.14%, 44.90%, 36.73%, 22.45%, 12.24%, and 8.16% of the isolates, respectively. Resistance to ampicillin (77.55%), oxacillin (69.39%), nalidixic acid (53.06%), amoxicillin (46.94%), oxytetracycline (46.94%), colistin sulfate (34.69%), fosfomycin (34.69%), chloramphenicol (32.65%), streptomycin (32.65%), cephalothin (28.57%), oxytetracycline (26.53%), ceftriaxone (20.41%), erythromycin (14.29%), and cefoxitin (12.24%) was detected in disc diffusion assay. Most of the isolates were classified as multidrug resistant as they scored multiple antimicrobial resistance index ≥0.2. Furthermore, antimicrobial resistance genes , , , , , , , , , , , and were found in 81.63%, 67.35%, 61.22%, 46.94%, 44.90%, 44.90%, 36.73%, 18.37%, 10.20%, 10.20%, 8.16% and 6.12% of the isolates, respectively. In conclusion, the development of antimicrobial resistance among will ultimately reduce the efficacy of antimicrobials used for treating and can favor the development of more virulent strains.
是一种重要的海洋动物和人类感染的原因。它在世界范围内的食源性感染中经常被报道。本研究旨在确定从韩国养殖的海洋食用鱼中分离出的对人类健康的潜在影响，研究其毒力和抗微生物药物耐药性。通过生化试验和多重 PCR 共分离了 49 株。通过表型检测毒力因子，结果显示有 81.63%的菌株具有 DNA 酶活性，75.51%和 12.25%的菌株具有溶血活性，71.43%的菌株具有明胶酶活性，71.43%的菌株具有蛋白酶产生能力，65.31%的菌株具有磷脂酶活性，34.69%的菌株具有脂肪酶产生能力。在分离株中，分别有 57.14%、44.90%、36.73%、22.45%、12.24%和 8.16%的菌株检测到 、 、 、 、 和 等毒力基因。分离株对氨苄西林（77.55%）、苯唑西林（69.39%）、萘啶酸（53.06%）、阿莫西林（46.94%）、氧四环素（46.94%）、硫酸粘菌素（34.69%）、磷霉素（34.69%）、氯霉素（32.65%）、链霉素（32.65%）、头孢噻吩（28.57%）、氧四环素（26.53%）、头孢曲松（20.41%）、红霉素（14.29%）和头孢西丁（12.24%）的耐药率较高。在纸片扩散试验中，大多数分离株被归类为多药耐药，因为它们的抗菌药物耐药指数（multiple antimicrobial resistance index，MARI）≥0.2。此外，在 81.63%、67.35%、61.22%、46.94%、44.90%、44.90%、36.73%、18.37%、10.20%、10.20%、8.16%和 6.12%的分离株中发现了 、 、 、 、 、 、 、 、 、 和 等抗菌药物耐药基因。总之， 对抗菌药物的耐药性的发展最终将降低用于治疗的抗菌药物的疗效，并有利于更具毒力的 菌株的发展。
