结核分枝杆菌基因分型的等位基因特异性寡核苷酸多重 PCR（ASO-PCR）方法。
Mycobacterium tuberculosis typing using Allele-specific oligonucleotide multiplex PCR (ASO-PCR) method.
机构信息
Infectious Diseases Research Center, Golestan University of Medical Sciences, 49178-67439, Gorgan, Iran.
Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran.
出版信息
Curr Microbiol. 2021 Dec;78(12):4009-4013. doi: 10.1007/s00284-021-02659-7. Epub 2021 Sep 27.
Mycobacterium tuberculosis (M. tuberculosis) genotyping provides valuable information related to the origin and the evolution of the isolates. This study aimed to evaluate the applicability of single-nucleotide polymorphisms (SNPs) technique for lineages identification of M. tuberculosis and compare it with mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR) method. The lineages of 162 clinically isolates were evaluated using six pair primers by Multiplex-PCR based on SNPs. Among 162 isolates, 70 (43.2%) isolates were lineage 4, following that 62 (38.3%) and 22 (13.6%) isolates were lineage 3 and 2, respectively. The method could not type 8 (4.9%) isolates. Moreover, we could identify 71 out of 79 unknown isolates resulted from the MIRU-VNTR method. The results showed that the SNP typing method has the potential to determine the lineages of M. tuberculosis as a rapid laboratory screening test.
结核分枝杆菌（M. tuberculosis）基因分型提供了与分离株起源和进化相关的有价值的信息。本研究旨在评估单核苷酸多态性（SNP）技术在结核分枝杆菌谱系鉴定中的适用性，并将其与分枝杆菌间隔重复可变数量串联重复（MIRU-VNTR）方法进行比较。通过基于 SNP 的多重 PCR 用六对引物评估了 162 株临床分离株的谱系。在 162 株分离株中，70 株（43.2%）为谱系 4，其次是 62 株（38.3%）和 22 株（13.6%）为谱系 3 和 2。该方法不能对 8 株（4.9%）分离株进行分型。此外，我们可以鉴定出 MIRU-VNTR 方法产生的 79 个未知分离株中的 71 个。结果表明，SNP 分型方法具有作为快速实验室筛选试验确定结核分枝杆菌谱系的潜力。
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