多哥/非洲用于治疗癌症的一些传统药用植物的抗氧化、抗血管生成和抗炎活性的民族药理学评价。
Ethnopharmacological evaluation of antioxidant, anti-angiogenic, and anti-inflammatory activity of some traditional medicinal plants used for treatment of cancer in Togo/Africa.
机构信息
Research Unit Pathophysiology-Bioactive Substances and Safety, Faculty of Sciences, University of Lome, 01 BP: 1515, Lome, Togo; Department of Pharmacology, JSS College of Pharmacy, JSS Academy of Higher Education and Research, Mysuru, 570015, India; Department of Pharmacognosy, JSS College of Pharmacy, JSS Academy of Higher Education and Research, Mysuru, 570015, India.
Research Unit Pathophysiology-Bioactive Substances and Safety, Faculty of Sciences, University of Lome, 01 BP: 1515, Lome, Togo.
出版信息
J Ethnopharmacol. 2022 Jan 30;283:114673. doi: 10.1016/j.jep.2021.114673. Epub 2021 Sep 25.
ETHNOPHARMACOLOGICAL RELEVANCE
Cancer is a multistep disease and its management is exceedingly expensive. Nowadays medicinal plants are gaining more attention in drug discovery and approximately 70% of anticancer drugs were developed from natural products or plants. A strong candidate from medicinal plant with anticancer potential should have four major properties: antioxidant, anti-inflammatory, anti-angiogenic, and cytotoxic activities.
AIM OF THE STUDY
In order to assess Togolese traditional healer's claims about the anticancer potential of medicinal plants and obtain candidate plants for anticancer drug discovery, some species were selected from surveys and evaluated for their antioxidant, anti-inflammatory, anti-angiogenic and cytotoxic activities.
METHODS
Four species, Cochlospermum planchonii (CP), Piliostigma thonningii (PT), Paullinia pinnata (PP), and Securidaca longipedunculata (SL) were selected and analyzed to detect the phytochemical components. The mentioned bioactivities were evaluated using in vitro, ex vivo and in vivo assays.
RESULTS
Relative to SL extract, CP and PT have shown significantly high polyphenols and flavonoids content. The DPPH, FRAP, and TAC of the extracts revealed that CP, PT, and PP have a potent antioxidant effect compared to SL. MDA analysis revealed the same antioxidant activity as CP, PT and PP showed a minor MDA level. The egg albumin denaturation assay showed that IC50 of CP and PP was significantly higher than control (P < 0.05). In contrast, the Bovine Serum Albumin (BSA) results showed a nonsignificant effect (P > 0.05). Notably, SL extract was nonsignificant to control in both Egg Albumin and BSA. Furthermore, angiogenesis assay showed that SL at 50 μg/ml and PP at 100 μg/ml effectively reduced the number of blood vessels than control and showed a potent anti-angiogenic effect (2.7-fold and 2.5-fold, respectively, P < 0.05). No cytotoxicity on PBMC was reported for CP, PP, and PT up to 1000 μg/ml, whereas SL at 1000 μg/ml exhibit benign cytotoxicity (P < 0.0001).
CONCLUSION
This study provided in vitro evidence supporting further evaluation on cancer cell lines and tumors in vivo.
民族药理学相关性
癌症是一种多步骤的疾病，其治疗费用非常昂贵。如今，药用植物在药物发现中受到了更多的关注，大约 70%的抗癌药物是从天然产物或植物中开发出来的。具有抗癌潜力的药用植物的一个强有力的候选者应该具有四大特性：抗氧化、抗炎、抗血管生成和细胞毒性。
研究目的
为了评估多哥传统治疗师关于药用植物抗癌潜力的说法，并获得抗癌药物发现的候选植物，从调查中选择了一些物种，并评估了它们的抗氧化、抗炎、抗血管生成和细胞毒性活性。
方法
选择了 Cochlospermum planchonii（CP）、Piliostigma thonningii（PT）、Paullinia pinnata（PP）和 Securidaca longipedunculata（SL）四种物种进行分析，以检测其植物化学成分。使用体外、离体和体内试验评估了上述生物活性。
结果
与 SL 提取物相比，CP 和 PT 表现出明显较高的多酚和类黄酮含量。提取物的 DPPH、FRAP 和 TAC 表明，CP、PT 和 PP 与 SL 相比具有较强的抗氧化作用。MDA 分析显示出相同的抗氧化活性，CP、PT 和 PP 显示出较低的 MDA 水平。卵清蛋白变性试验表明，CP 和 PP 的 IC50 明显高于对照（P<0.05）。相比之下，牛血清白蛋白（BSA）结果显示无显著影响（P>0.05）。值得注意的是，SL 提取物在卵清蛋白和 BSA 中均与对照无显著差异。此外，血管生成试验表明，SL 在 50μg/ml 和 PP 在 100μg/ml 时可有效减少血管数量，表现出强大的抗血管生成作用（分别为 2.7 倍和 2.5 倍，P<0.05）。CP、PP 和 PT 在高达 1000μg/ml 时对 PBMC 无细胞毒性，而 SL 在 1000μg/ml 时表现出良性细胞毒性（P<0.0001）。
结论
本研究提供了体外证据，支持进一步在癌细胞系和体内肿瘤上进行评估。
Zotero 插件