Strickland Jaimie M, Wisnieski Lauren, Mavangira Vengai, Sordillo Lorraine M
Large Animal Clinal Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA.
Center for Animal and Human Health in Appalachia, College of Veterinary Medicine, Lincoln Memorial University, Harrogate, TN 37752, USA.
Antioxidants (Basel). 2021 Sep 6;10(9):1420. doi: 10.3390/antiox10091420.
Dairy cows experience increased oxidative stress during periods of transition such as at the cessation of lactation and around the periparturient period, thus increasing disease risk. Despite routine supplementation of transition cow diets with certain vitamins in an attempt to mitigate oxidative stress, there is no currently available data directly linking vitamin supplementation with antioxidant potential (AOP) in transition cows. The objective of this study was to determine the association between serum vitamins and biomarkers of oxidative stress in healthy cows. Blood samples were collected from 240 cows at dry off (DO), close up (CU), and 2-10 days post-calving (DIM2-10). Blood samples were analyzed for vitamins (A, D, E), β-carotene, reactive oxygen species (ROS), and AOP. Spearman correlations and mixed linear regression models were used to assess associations between vitamins and measures of oxidant status. Vitamin D concentrations were positively associated with AOP at the CU and DIM2-10. Based on the positive association with AOP, additional in-vitro studies were conducted that showed vitamin D mitigated barrier integrity loss in endothelial cells during oxidative stress. These results indicate for the first time that vitamin D may have a role in promoting antioxidant potential in transition dairy cows.
奶牛在过渡时期，如泌乳停止时和围产期前后，会经历氧化应激增加的情况，从而增加患病风险。尽管常规给过渡奶牛日粮补充某些维生素以减轻氧化应激，但目前尚无直接将维生素补充与过渡奶牛抗氧化潜力（AOP）联系起来的数据。本研究的目的是确定健康奶牛血清维生素与氧化应激生物标志物之间的关联。在干奶期（DO）、围产前期（CU）和产后2 - 10天（DIM2 - 10）从240头奶牛采集血样。对血样进行维生素（A、D、E）、β-胡萝卜素、活性氧（ROS）和AOP分析。使用Spearman相关性和混合线性回归模型评估维生素与氧化状态指标之间的关联。在围产前期和产后2 - 10天，维生素D浓度与AOP呈正相关。基于与AOP的正相关，进行了额外的体外研究，结果表明维生素D可减轻氧化应激期间内皮细胞屏障完整性的损失。这些结果首次表明维生素D可能在促进过渡奶牛抗氧化潜力方面发挥作用。
