Yin Lanmei, Yang Qing, Zhang Yiming, Wan Dan, Yin Yuebang, Wang Qiye, Huang Jing, Li Jianzhong, Yang Huansheng, Yin Yulong
Laboratory of Animal Nutrition and Human Health, Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.
Animals (Basel). 2021 Aug 26;11(9):2513. doi: 10.3390/ani11092513.
Copper (Cu) is an essential micronutrient for animals. Many studies have been conducted on the effects of dietary Cu on growth performance, intestinal morphology, and function of piglets. However, the underlying mechanism remains to be explored. Intestinal stem cells (ISC) drive the development and constant renewal of intestinal epithelium. Therefore, we hypothesized that dietary Cu affects piglets' intestinal development via modulating ISC activity. A total of eighty-five 21-day-old piglets were randomly assigned to five groups, where 25, 50, 75, 100, and 125 mg CuSO/kg on a dry matter basis were supplemented to the basal diet at phase 1 (day 0 to 21). Increasing the dietary Cu concentration decreased ( < 0.05) villus width but increased ( < 0.001) the number of Ki67-positive cells. At phase 2 (day 22 to 163), the other 45 pigs were offered the same diets. Villus height in the 125 mg/kg Cu group was greater ( < 0.001) than in the other groups. Moreover, the effects of Cu on ISC activity in vitro were tested to explore the underlying mechanism. Compared to the control group, 10 μmol/L CuSO·5HO increased ( < 0.001) the organoid budding efficiency, crypt depth, and crypts per organoid. Dietary Cu improved the intestinal morphology of finishing pigs via promoting cell proliferation and modulating ISC activity.
铜（Cu）是动物必需的微量营养素。关于日粮铜对仔猪生长性能、肠道形态和功能的影响，已经开展了许多研究。然而，其潜在机制仍有待探索。肠道干细胞（ISC）驱动肠道上皮的发育和持续更新。因此，我们假设日粮铜通过调节ISC活性影响仔猪的肠道发育。总共85头21日龄仔猪被随机分为五组，在第1阶段（第0至21天），以干物质计，在基础日粮中分别添加25、50、75、100和125 mg CuSO₄/kg。增加日粮铜浓度可降低（P<0.05）绒毛宽度，但增加（P<0.001）Ki67阳性细胞数量。在第2阶段（第22至163天），另外45头猪饲喂相同的日粮。125 mg/kg铜组的绒毛高度高于（P<0.001）其他组。此外，测试了铜对体外ISC活性的影响以探索潜在机制。与对照组相比，10 μmol/L CuSO₄·5H₂O提高了（P<0.001）类器官出芽效率、隐窝深度和每个类器官的隐窝数量。日粮铜通过促进细胞增殖和调节ISC活性改善了育肥猪的肠道形态。
