Gori Eleonora, Pierini Alessio, Nesci Martina, Benvenuti Elena, Tasca Silvia, Lubas George, Marchetti Veronica
Veterinary Teaching Hospital "Mario Modenato", Department of Veterinary Sciences, University of Pisa, 56124 Pisa, Italy.
Clinical Pathology Division, San Marco Veterinary Clinic and Laboratory, 35030 Padova, Italy.
Animals (Basel). 2021 Sep 2;11(9):2580. doi: 10.3390/ani11092580.
Several extra-intestinal manifestations, including immune-mediated cytopenias, are reported in human inflammatory bowel disease (IBD), whereas they are poorly documented in dogs. Hypothesizing that immune-mediated subclinical anemia can occur in canine IBD, the study aim was to evaluate the erythrogram and the presence of anti-RBC antibodies in dogs with IBD. IBD was diagnosed according to the following criteria: chronic gastrointestinal signs, ruling out of extra-intestinal diseases, no improvement with diet trial, histological evidence of inflammatory infiltration, and improvement after immunosuppressant therapy. Canine Chronic Enteropathy Clinical Activity Index (CCECAI) endoscopic and histopathological scores were assessed for each dog. Twenty-five dogs were enrolled, and each dog had a CBC evaluation prior to endoscopy. The CBC was performed using laser hematology analyzer and blood smears were carefully reviewed for the presence of nucleated RBC, anisocytosis, polychromasia, and Howell-Jolly bodies. IgG and IgM anti-RBC antibodies were evaluated with flow cytometry. A high frequency of positive cases for anti-RBC antibodies in dogs with IBD (17/25 dogs) was ascertained. Approximatively 50% of dogs showed some hematologic features of RBC regeneration in addition to hematologic findings consistent with chronic inflammation. Anti-RBC antibodies and signs of erythroid regeneration may suggest possible subclinical chronic immune-mediated hemolysis that can cause anemia in dogs with IBD, together with the chronic inflammation.
在人类炎症性肠病（IBD）中，有多种肠外表现被报道，包括免疫介导的血细胞减少症，而在犬类中相关记录较少。基于免疫介导的亚临床贫血可能发生在犬IBD中的假设，本研究旨在评估患有IBD的犬的血常规及抗红细胞抗体的存在情况。IBD根据以下标准进行诊断：慢性胃肠道症状，排除肠外疾病，饮食试验无改善，有炎症浸润的组织学证据，以及免疫抑制治疗后改善。对每只犬评估犬慢性肠病临床活动指数（CCECAI）内镜和组织病理学评分。共纳入25只犬，每只犬在进行内镜检查前均进行了血常规评估。血常规使用激光血液分析仪进行检测，并仔细检查血涂片以确定有无有核红细胞、红细胞大小不均、嗜多色性红细胞和豪-焦小体。通过流式细胞术评估IgG和IgM抗红细胞抗体。已确定患有IBD的犬中抗红细胞抗体阳性病例的频率较高（25只犬中有17只）。除了与慢性炎症一致的血液学表现外，约50%的犬还表现出一些红细胞再生的血液学特征。抗红细胞抗体和红细胞生成迹象可能提示可能存在亚临床慢性免疫介导的溶血，这可能与慢性炎症一起导致患有IBD的犬出现贫血。
