Rosa Fernanda, Michelotti Tainara Cristina, St-Pierre Benoit, Trevisi Erminio, Osorio Johan S
Department of Dairy and Food Sciences, South Dakota State University, Brookings, SD 57007, USA.
Department of Animal Sciences, South Dakota State University, Brookings, SD 57007, USA.
Animals (Basel). 2021 Sep 15;11(9):2704. doi: 10.3390/ani11092704.
This study aimed to evaluate the effects of early life fecal microbiota transplantation (FMT) on the health and performance of neonatal dairy calves. The donor was selected based on health and production records and fecal material testing negative for infectious pathogens. Sixteen healthy newborn Holstein calves were randomized to either a baseline nutritional program (CON) or 1×/d inoculations with 25 g of fecal donor material (FMT) mixed in the milk replacer (n = 8/TRT) from 8 to 12 days of age. Blood and fecal samples were collected weekly, and calves were weaned at 7 weeks of age. A TRT × Week interaction was observed in haptoglobin, which was reflected in a positive quadratic effect in FMT calves but not in CON. A trend for a TRT × Week interaction was observed in the liver function biomarker paraoxonase, which resulted in greater paraoxonase in FMT calves than CON at three weeks of age. Fecal microbial community analysis revealed a significant increase in the alpha-diversity between week 1 and week 5 for the FMT calves. These results suggest that early life FMT in neonatal calves has positive effects in mediating the inflammatory response and gut microbial maturation.
本研究旨在评估早期粪便微生物群移植（FMT）对新生奶牛犊健康和性能的影响。根据健康和生产记录以及粪便材料检测传染性病原体呈阴性来选择供体。16头健康的新生荷斯坦犊牛被随机分为接受基线营养方案（CON）组或从8日龄至12日龄每天1次接种25克粪便供体材料（FMT）并混入代乳粉中的组（每组n = 8）。每周采集血液和粪便样本，犊牛在7周龄时断奶。在触珠蛋白中观察到TRT×周的交互作用，这在FMT犊牛中表现为正二次效应，而在CON组中未观察到。在肝功能生物标志物对氧磷酶中观察到TRT×周交互作用的趋势，这导致FMT犊牛在3周龄时的对氧磷酶含量高于CON组。粪便微生物群落分析显示，FMT犊牛在第1周和第5周之间的α多样性显著增加。这些结果表明，新生犊牛的早期FMT在介导炎症反应和肠道微生物成熟方面具有积极作用。
