International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, 468-1 Aoba, Aramaki, Aoba-Ku, Sendai, Miyagi, 980-8572, Japan.
Laboratory of Animal Functional Morphology, Graduate School of Agricultural Science, Tohoku University, Miyagi, 980-8572, Japan.
Sci Rep. 2024 Nov 14;14(1):28078. doi: 10.1038/s41598-024-79267-5.
Fecal microbiota transplantation (FMT) is a therapeutic modality for treating neonatal calf diarrhea. Several practical barriers, including donor selection, fecal collection, and a limited timeframe for FMT, are the main constraints to using fresh feces for implementing on-farm FMT. We report the utility of FMT with pretreated ready-to-use frozen (F) or freeze-dried (FD) microorganisms for treating calf diarrhea. In total, 19 FMT (F-FMT, n = 10 and FD-FMT, n = 9) treatments were conducted. Both FMT treatments were 100% clinically effective; however, multi-omics analysis showed that FD-FMT was superior to F-FMT. Machine learning analysis with SourceTracker confirmed that donor microbiota was retained four times better in the recipient calves treated with FD-FMT than F-FMT. A predictive model based on receiver operating characteristic curve analysis and area under the curve showed that FD-FMT was more discriminative than F-FMT of the observed changes in microbiota and metabolites during disease recovery. These results provide new insights into establishing methods for preparing fecal microorganisms to increase the quality of FMT in animals and may contribute to FMT in humans.
粪便微生物群移植（FMT）是治疗新生犊牛腹泻的一种治疗方式。在农场实施 FMT 时，使用新鲜粪便存在一些实际障碍，包括供体选择、粪便采集和 FMT 的有限时间窗口。我们报告了使用预处理即用型冷冻（F）或冻干（FD）微生物进行 FMT 治疗犊牛腹泻的效用。总共进行了 19 次 FMT（F-FMT，n=10 和 FD-FMT，n=9）治疗。两种 FMT 治疗均 100%具有临床疗效；然而，多组学分析表明 FD-FMT 优于 F-FMT。使用 SourceTracker 的机器学习分析证实，FD-FMT 处理的受体小牛中供体微生物群的保留率比 F-FMT 高 4 倍。基于接收者操作特征曲线分析和曲线下面积的预测模型表明，FD-FMT 比 F-FMT 更能区分疾病恢复过程中微生物群和代谢物观察到的变化。这些结果为制备粪便微生物的方法提供了新的见解，以提高动物 FMT 的质量，并可能有助于人类的 FMT。
