Avila-Novoa María Guadalupe, Navarrete-Sahagún Velia, González-Gómez Jean Pierre, Novoa-Valdovinos Carolina, Guerrero-Medina Pedro Javier, García-Frutos Ramón, Martínez-Chávez Liliana, Martínez-Gonzáles Nanci Edid, Gutiérrez-Lomelí Melesio
Centro de Investigación en Biotecnología Microbiana y Alimentaria, Departamento de Ciencias Básicas, División de Desarrollo Biotecnológico, Centro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad 1115, Ocotlán 47810, Jalisco, Mexico.
Laboratorio Nacional para la Investigación en Inocuidad Alimentaria (LANIIA), Centro de Investigación en Alimentación y Desarrollo A.C. (CIAD), Carretera a Eldorado Km. 5.5, Culiacán 80110, Sinaloa, Mexico.
Foods. 2021 Sep 5;10(9):2097. doi: 10.3390/foods10092097.
is an important pathogen that has been implicated in foodborne illnesses and the recall of products such as fruit and vegetables. This study determines the prevalence of virulence-associated genes and serogroups and evaluates the effects of different growth media and environmental conditions on biofilm formation by . Eighteen isolates from Hass avocados sold at markets in Guadalajara, Mexico, were characterized by virulence-associated genes and serogroup detection with PCR. All isolates harbored 88.8% , 88.8% , 83.3% , 77.7% , 77.7% , 66.6% , 55.5% , and 33.3% . The results showed that 38.8% of isolates harbored virulence genes belonging to pathogenicity island 1 (LIPI-1). PCR revealed that the most prevalent serogroup was serogroup III (1/2b, 3b, and 7 ( = 18, 66.65%)), followed by serogroup IV (4b, 4d-4e ( = 5, 27.7%)) and serogroup I (1/2a-3a ( = 1, 5.5%)). The assessment of the ability to develop biofilms using a crystal violet staining method revealed that responded to supplement medium TSBA, 1/10 diluted TSBA, and TSB in comparison with 1/10 diluted TSB ( < 0.05) on polystyrene at 240 h ( < 0.05). In particular, the biofilm formation by (7.78 ± 0.03-8.82 ± 0.03 log CFU/cm) was significantly different in terms of TSBA on polypropylene type B (PP) ( < 0.05). In addition, visualization by epifluorescence microscopy, scanning electron microscopy (SEM), and treatment (DNase I and proteinase K) revealed the metabolically active cells and extracellular polymeric substances of biofilms on PP. has the ability to develop biofilms that harbor virulence-associated genes, which represent a serious threat to human health and food safety.
是一种重要的病原体，与食源性疾病以及水果和蔬菜等产品的召回有关。本研究确定了毒力相关基因和血清群的流行情况，并评估了不同生长培养基和环境条件对其生物膜形成的影响。对墨西哥瓜达拉哈拉市场销售的哈斯鳄梨中分离出的18株菌株进行了毒力相关基因鉴定和血清群检测，并采用PCR方法进行分析。所有分离株携带88.8%的[具体基因1]、88.8%的[具体基因2]、83.3%的[具体基因3]、77.7%的[具体基因4]、77.7%的[具体基因5]、66.6%的[具体基因6]、55.5%的[具体基因7]和33.3%的[具体基因8]。结果表明，38.8%的分离株携带属于1型致病岛（LIPI-1）的毒力基因。PCR检测显示，最常见的血清群是血清群III（1/2b、3b和7（n = 18，66.65%）），其次是血清群IV（4b、4d - 4e（n = 5，27.7%））和血清群I（1/2a - 3a（n = 1，5.5%））。采用结晶紫染色法评估生物膜形成能力的结果显示，与240小时聚苯乙烯上的1/10稀释胰蛋白胨大豆肉汤（TSB）相比，[该病原体名称]对补充培养基TSBA、1/10稀释TSBA和TSB有反应（P < 0.05）。特别是，在B型聚丙烯（PP）上，[该病原体名称]在TSBA条件下形成生物膜的情况（7.78 ± 0.03 - 8.82 ± 0.03 log CFU/cm）与其他条件相比有显著差异（P < 0.05）。此外，通过落射荧光显微镜、扫描电子显微镜（SEM）观察以及（脱氧核糖核酸酶I和蛋白酶K）处理，揭示了PP上生物膜的代谢活跃细胞和细胞外聚合物。[该病原体名称]具有形成携带毒力相关基因的生物膜的能力，这对人类健康和食品安全构成严重威胁。
