Velderrain-Rodríguez Gustavo R, Salvia-Trujillo Laura, Martín-Belloso Olga
Department of Food Technology, University of Lleida-Agrotecnio Center, 25198 Lleida, Spain.
Foods. 2021 Sep 16;10(9):2193. doi: 10.3390/foods10092193.
In this study, the digestibility of oil-in-water (O/W) emulsions using low methoxyl pectin (LMP) as surfactant and in combination with avocado peel (AP) or seed (AS) extracts was assessed, in terms of its free fatty acid (FFA) release and the phenolic compound (PC) bioaccessibility. With this purpose, AP and AS were characterized by UPLC-ESI-MS/MS before their incorporation into O/W emulsions stabilized using LMP. In that sense, AP extract had a higher content of PCs (6836.32 ± 64.66 mg/100 g of extract) compared to AS extract (1514.62 ± 578.33 mg/100 g of extract). Both extracts enhanced LMP's emulsifying properties, leading to narrower distributions and smaller particle sizes compared to those without extracts. Similarly, when both LMP and the extracts were present in the emulsions the FFA release significantly increased. Regarding bioaccessibility, the PCs from the AS extracts had a higher bioaccessibility than those from the AP extracts, regardless of the presence of LMP. However, the presence of LMP reduced the bioaccessibility of flavonoids from emulsions containing either AP or AS extracts. These results provide new insights regarding the use of PC extracts from avocado peel and seed residues, and the effect of LMP on emulsion digestibility, and its influence on flavonoids bioaccessibility.
在本研究中，评估了以低甲氧基果胶（LMP）作为表面活性剂并与鳄梨皮（AP）或种子（AS）提取物组合的水包油（O/W）乳液的消化率，包括其游离脂肪酸（FFA）释放和酚类化合物（PC）的生物可及性。为此，在将AP和AS纳入用LMP稳定的O/W乳液之前，通过超高效液相色谱-电喷雾串联质谱（UPLC-ESI-MS/MS）对其进行了表征。从这个意义上说，与AS提取物（1514.62±578.33 mg/100 g提取物）相比，AP提取物的PC含量更高（6836.32±64.66 mg/100 g提取物）。两种提取物均增强了LMP的乳化性能，与不含提取物的乳液相比，粒径分布更窄且粒径更小。同样，当乳液中同时存在LMP和提取物时，FFA释放显著增加。关于生物可及性，无论LMP是否存在，AS提取物中的PCs比AP提取物中的PCs具有更高的生物可及性。然而，LMP的存在降低了含有AP或AS提取物的乳液中黄酮类化合物的生物可及性。这些结果为鳄梨皮和种子残渣中PC提取物的使用、LMP对乳液消化率的影响及其对黄酮类化合物生物可及性的影响提供了新的见解。
