Hwang Hee-Jeong, Lee Sae-Ha, Kim Yong-Yeon, Shin Han-Seung
Research Institute of Biotechnology and Medical Converged Science, Dongguk University-Seoul, 32, Dongguk-ro, Ilsandong-gu, Goyang-si 10326, Gyeonggi-do, Korea.
Department of Food Science and Biotechnology, Dongguk University-Seoul, 32, Dongguk-ro, Ilsandong-gu, Goyang-si 10326, Gyeonggi-do, Korea.
Foods. 2021 Sep 16;10(9):2200. doi: 10.3390/foods10092200.
Polycyclic aromatic hydrocarbons (PAHs) are carcinogenic and mutagenic compounds that are often formed during the thermal processing of herbal medicine ingredients. In this study, the concentrations of four PAHs (PAH4) in various herbal medicine ingredients were monitored. Further, the QuEChERS method was used to replace conventional pretreatment, a more complex and cumbersome approach. The recovery range of the QuEChERS method ranged between 89.65-118.59%, and the average detection levels of benzo[a]anthracene (BaA), chrysene (CHR), benzo[b]fluoranthene (BbF), and Benzo[a]pyrene (BaP) in 50 herbal medicine ingredients were 0.18, 0.27, 1.13, and 0.17 μg/kg, respectively. The BaP and PAH4 levels in all tested samples were deemed safe according to risk characterization analyses based on European Union and Korean guidelines. Therefore, our findings indicated that the QuEChERS method could be used as an effective alternative to conventional sample pretreatment for the analysis of herbal medicine ingredients.
多环芳烃（PAHs）是具有致癌性和致突变性的化合物，常在草药成分的热加工过程中形成。在本研究中，监测了各种草药成分中四种多环芳烃（PAH4）的浓度。此外，采用了QuEChERS方法替代传统的预处理方法，传统方法更为复杂繁琐。QuEChERS方法的回收率范围在89.65 - 118.59%之间，50种草药成分中苯并[a]蒽（BaA）、屈（CHR）、苯并[b]荧蒽（BbF）和苯并[a]芘（BaP）的平均检测水平分别为0.18、0.27、1.13和0.17 μg/kg。根据基于欧盟和韩国指南的风险特征分析，所有测试样品中的BaP和PAH4水平被认为是安全的。因此，我们的研究结果表明，QuEChERS方法可作为分析草药成分时传统样品预处理的有效替代方法。
