Abel P P, Nelson R S, De B, Hoffmann N, Rogers S G, Fraley R T, Beachy R N
Science. 1986 May 9;232(4751):738-43. doi: 10.1126/science.3457472.
A chimeric gene containing a cloned cDNA of the coat protein (CP) gene of tobacco mosaic virus (TMV) was introduced into tobacco cells on a Ti plasmid of Agrobacterium tumefaciens from which tumor inducing genes had been removed. Plants regenerated from transformed cells expressed TMV mRNA and CP as a nuclear trait. Seedlings from self-fertilized transgenic plants were inoculated with TMV and observed for development of disease symptoms. The seedlings that expressed the CP gene were delayed in symptom development and 10 to 60 percent of the transgenic plants failed to develop symptoms for the duration of the experiments. Increasing the concentration of TMV in the inoculum shortened the delay in appearance of symptoms. The results of these experiments indicate that plants can be genetically transformed for resistance to virus disease development.
一个含有烟草花叶病毒（TMV）外壳蛋白（CP）基因克隆cDNA的嵌合基因，通过去除致瘤基因的根癌农杆菌Ti质粒导入烟草细胞。从转化细胞再生的植株将TMV mRNA和CP作为核性状表达。对自花授粉转基因植株的幼苗接种TMV，并观察疾病症状的发展。表达CP基因的幼苗症状发展延迟，10%至60%的转基因植株在实验期间未出现症状。增加接种物中TMV的浓度缩短了症状出现的延迟时间。这些实验结果表明，植物可以通过基因转化获得对病毒病发展的抗性。
