Wang Daniel C, Clark Justin R, Lee Richard, Nelson Adam H, Maresso Anthony W, Acharya Ghanashyam, Shin Crystal S
Department of Surgery, Baylor College of Medicine, Houston, TX 77030, USA.
Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA.
Pharmaceutics. 2021 Sep 10;13(9):1445. doi: 10.3390/pharmaceutics13091445.
Nitric oxide (NO) is a highly reactive gas molecule, exhibiting antimicrobial properties. Because of its reactive nature, it is challenging to store and deliver NO efficiently as a therapeutic agent. The objective of this study was to develop NO-releasing polymeric fibers (NO-fibers), as an effective delivery platform for NO. NO-fibers were fabricated with biopolymer solutions of polyvinyl pyrrolidone (PVP) and ethylcellulose (EC), and derivatives of -diazeniumdiolate (NONOate) as NO donor molecules, using an electrospinning system. We evaluated in vitro NO release kinetics, along with antimicrobial effects and cytotoxicity in microorganisms and human cell culture models. We also studied the long-term stability of NONOates in NO-fibers over 12 months. We demonstrated that the NO-fibers could release NO over 24 h, and showed inhibition of the growth of Pseudomonas aeruginosa (P. aeruginosa) and methicillin-resistant Staphylococcus aureus (MRSA), without causing cytotoxicity in human cells. NO-fibers were able to store NONOates for over 12 months at room temperature. This study presents the development of NO-fibers, and the feasibility of NO-fibers to efficiently store and deliver NO, which can be further developed as a bandage.
一氧化氮（NO）是一种具有高反应活性的气体分子，具有抗菌特性。由于其反应活性，将NO作为治疗剂进行有效储存和递送具有挑战性。本研究的目的是开发可释放NO的聚合物纤维（NO纤维），作为一种有效的NO递送平台。使用静电纺丝系统，将聚乙烯吡咯烷酮（PVP）和乙基纤维素（EC）的生物聚合物溶液与作为NO供体分子的重氮二醇盐（NONOate）衍生物制成NO纤维。我们评估了体外NO释放动力学，以及在微生物和人类细胞培养模型中的抗菌效果和细胞毒性。我们还研究了NONOate在NO纤维中12个月以上的长期稳定性。我们证明，NO纤维可以在24小时内释放NO，并显示出对铜绿假单胞菌（P. aeruginosa）和耐甲氧西林金黄色葡萄球菌（MRSA）生长的抑制作用，而不会对人类细胞产生细胞毒性。NO纤维能够在室温下将NONOate储存12个月以上。本研究介绍了NO纤维的开发，以及NO纤维有效储存和递送NO的可行性，其可进一步开发为绷带。
