Meng Lu-Lu, Liu Rui-Cheng, Yang Liu, Zou Ying-Ning, Srivastava Anoop Kumar, Kuča Kamil, Hashem Abeer, Abd Allah Elsayed Fathi, Giri Bhoopander, Wu Qiang-Sheng
College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China.
ICAR-Central Citrus Research Institute, Nagpur 440033, Maharashtra, India.
J Fungi (Basel). 2021 Aug 31;7(9):716. doi: 10.3390/jof7090716.
Endophytes have the ability to improve plant nutrition alongside their agronomic performance, among which arbuscular mycorrhizal fungi provide the most benefits to their host. Previously, we reported for the first time that an arbuscular mycorrhizal-like fungus had the ability to colonize roots of trifoliate orange () and conferred positive effects on nutrient acquisition. Present study showed the changes in fatty acids and sugars to unravel the physiological and symbiotic association of trifoliate orange with . and an arbuscular mycorrhizal fungus, singly or in combination. All the endophytic fungi collectively increased fructose, glucose, and sucrose content in leaves and roots, along with a relatively higher increase with . inoculation than with . alone or dual inoculation. Treatment with . increased the concentration of part unsaturated fatty acids such as C18:3N6, C20:2, C20:3N6, C20:4N6, C20:3N3, C20:5N3, C22:1N9, and C24:1. Additionally, . induced the increase in the concentration of part saturated fatty acids such as C6:0, C8:0, C13:0, C14:0, and C24:0. . hardly changed the content of fatty acids, except for increase in C14:0 and C20:5N3. Double inoculation only reduced the C21:0, C10:0, C12:0, C18:3N3, and C18:1 content and increased the C20:5N3 content. These endophytic fungi up-regulated the root , and gene expression level, coupled with a higher expression of and by . than by . . It was concluded that . exhibited a stronger response, for sugars and fatty acids, than . on trifoliate orange. Such results also reveal the (an culturable fungus) as a bio-stimulator applying to citriculture.
内生菌具有改善植物营养及其农艺性能的能力，其中丛枝菌根真菌对其宿主的益处最大。此前，我们首次报道了一种类丛枝菌根真菌能够定殖于枳（）根系，并对养分获取产生积极影响。本研究通过分析脂肪酸和糖类的变化，以揭示枳与该类丛枝菌根真菌单独或组合共生时的生理及共生关系。所有内生真菌均使叶片和根系中的果糖、葡萄糖和蔗糖含量增加，其中接种该类丛枝菌根真菌时的增幅相对高于单独接种或双重接种另一类丛枝菌根真菌时的增幅。接种该类丛枝菌根真菌增加了部分不饱和脂肪酸的浓度，如C18:3N6、C20:2、C20:3N6、C20:4N6、C20:3N3、C20:5N3、C22:1N9和C24:1。此外，该类丛枝菌根真菌还诱导了部分饱和脂肪酸浓度的增加，如C6:0、C8:0、C13:0、C14:0和C24:0。另一类丛枝菌根真菌除了使C14:0和C20:5N3含量增加外，几乎未改变脂肪酸含量。双重接种仅降低了C21:0、C10:0、C12:0、C18:3N3和C18:1的含量，并增加了C20:5N3的含量。这些内生真菌上调了根系、和基因的表达水平，且该类丛枝菌根真菌接种时和的表达量高于另一类丛枝菌根真菌接种时。研究得出结论，该类丛枝菌根真菌在枳的糖类和脂肪酸方面表现出比另一类丛枝菌根真菌更强的响应。这些结果还表明该类可培养真菌作为一种生物刺激剂应用于柑橘栽培。
