Cao Ming-Ao, Liu Rui-Cheng, Xiao Zhi-Yan, Hashem Abeer, Abd Allah Elsayed Fathi, Alsayed Mashail Fahad, Harsonowati Wiwiek, Wu Qiang-Sheng
College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China.
Wuhan Forestry Workstation, Wuhan 430023, China.
J Fungi (Basel). 2022 Jul 29;8(8):800. doi: 10.3390/jof8080800.
Plant roots can be colonized by many symbiotic fungi, whereas it is unclear whether and how symbiotic fungi including arbuscular mycorrhizal fungi and endophytic fungi promote phosphorus (P) uptake in plants. The objective of the present study was to analyze the effect of inoculation with a culturable endophytic fungus (), three arbuscular mycorrhizal fungi (, , and ), and mixture of . , . and . on plant growth, root architecture, soil Olsen-P, soil phosphatase activities, leaf and root P concentrations, and phosphate transporter gene expressions, in order to explore the potential and mechanism of these symbiotic fungi on P acquisition. All the symbiotic fungi colonized roots of . after 16 weeks, with . showing the best effect on fungal colonization. All the symbiotic fungi significantly increased acid, neutral, and total phosphatase activities in the soil, accompanied with an elevation of soil Olsen-P, of which . presented the best effect. All symbiotic fungal treatments, except . , significantly promoted plant growth, coupled with an increase in root total length, area, and volume. Symbiotic fungi almost up-regulated root expressions as well as leaf , , and expressions. Correlation analysis showed that P concentrations in leaves and roots were significantly positively correlated with root morphological variables (length, volume, and surface area) and soil acid, neutral and total phosphatase activities. It is concluded that symbiotic fungi, especially . , played an important role in P uptake of . plants through regulating root architecture, part plant phosphate transporter gene expressions and soil phosphatase activities.
植物根系可被多种共生真菌定殖，然而包括丛枝菌根真菌和内生真菌在内的共生真菌是否以及如何促进植物对磷（P）的吸收尚不清楚。本研究的目的是分析接种一种可培养的内生真菌（）、三种丛枝菌根真菌（、和）以及.、. 和. 的混合物对植物生长、根系结构、土壤有效磷、土壤磷酸酶活性、叶片和根系磷浓度以及磷酸盐转运蛋白基因表达的影响，以探索这些共生真菌在磷获取方面的潜力和机制。16周后，所有共生真菌都定殖在. 的根系上，其中. 对真菌定殖的效果最佳。所有共生真菌均显著提高了土壤中的酸性、中性和总磷酸酶活性，同时土壤有效磷也有所升高，其中. 的效果最佳。除. 外，所有共生真菌处理均显著促进了植物生长，同时根系总长度、面积和体积增加。共生真菌几乎上调了根系的表达以及叶片、和的表达。相关性分析表明，叶片和根系中的磷浓度与根系形态变量（长度、体积和表面积）以及土壤酸性、中性和总磷酸酶活性显著正相关。研究得出结论，共生真菌，尤其是.，通过调节根系结构、部分植物磷酸盐转运蛋白基因表达和土壤磷酸酶活性，在. 植物的磷吸收中发挥了重要作用。
