Chen S F, Gryzenhout M, Roux J, Xie Y J, Wingfield M J, Zhou X D
Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa, and China Eucalypt Research Centre (CERC), Chinese Academy of Forestry (CAF), China.
Department of Microbiology and Plant Pathology, FABI.
Plant Dis. 2010 Sep;94(9):1143-1150. doi: 10.1094/PDIS-94-9-1143.
The genus Chrysoporthe includes important pathogens of plantation-grown Eucalyptus spp. and has been reported from several tree genera in the order Myrtales in tropical and subtropical areas of the world. During disease surveys in South China, fruiting structures typical of Chrysoporthe spp. were observed on cankers on Eucalyptus spp. and Syzygium cumini trees. The aim of this study was to confirm the identity of the Chrysoporthe cubensis infecting the Eucalyptus spp. and S. cumini and to test the pathogenicity of the fungus. Following glasshouse trials to select virulent isolates, field inoculations were undertaken to screen different commercial Eucalyptus genotypes for their susceptibility to the fungus. Isolates were characterized based on their morphology and DNA sequence data for the β-tubulin and internal transcribed spacer regions of the ribosomal DNA. Results showed that the putative pathogen represented the Asian form of C. cubensis, which occurred on numerous different Eucalyptus spp. and hybrid clones as well as S. cumini. Field inoculations showed that all six of the Eucalyptus genotypes tested are susceptible to infection by C. cubensis. Significant differences were observed between them, providing prospects to select disease-tolerant planting stock in the future.
金梭孢属包含人工种植的桉属植物的重要病原菌，并且在世界热带和亚热带地区桃金娘目下的几个树木属中都有报道。在中国南方的病害调查期间，在桉属植物和莲雾树的溃疡病斑上观察到了典型的金梭孢属产果结构。本研究的目的是确认感染桉属植物和莲雾的古巴金梭孢的身份，并测试该真菌的致病性。在温室试验中筛选出强毒株后，进行了田间接种，以筛选不同商业桉属基因型对该真菌的易感性。根据分离株的形态以及核糖体DNA的β微管蛋白和内转录间隔区的DNA序列数据对其进行了鉴定。结果表明，推定的病原菌代表古巴金梭孢的亚洲型，它出现在许多不同的桉属植物、杂交克隆以及莲雾上。田间接种表明，测试的所有六种桉属基因型都易受古巴金梭孢感染。在它们之间观察到了显著差异，为未来选择抗病种植材料提供了前景。
