Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, Jilin University, Changchun 130012, China.
School of Life Sciences, Jilin University, Changchun 130012, China.
Int J Mol Sci. 2021 Sep 7;22(18):9668. doi: 10.3390/ijms22189668.
is a valuable traditional Chinese medicine in Northeast China. Ginsenoside, the active component of ginseng, has not been investigated much for its effects on aging and its underlying mechanism(s) of action. Here, we investigated the effects of total ginsenoside (TG), a mixture of the primary active ginsenosides from , on the lifespan of (). We found that TG extended the lifespan of and reduced lipofuscin accumulation. Moreover, TG increased the survival of in response to heat and oxidative stress via the reduction of ROS. Next, we used RNA-seq to fully define the antiaging mechanism(s) of TG. The KEGG pathway analysis showed that TG can prolong the lifespan and is involved in the longevity regulating pathway. qPCR showed that TG upregulated the expression of , , , and their downstream genes. TG also reduced the fat accumulation and promoted lipid metabolism. Moreover, TG failed to extend the lifespan of and mutants, highlighting their role in the antiaging effects of TG in . The four main constitution of TG were then confirmed by HPLC and included ginsenoside Re, Rg, Rg and Rd. Of the ginsenosides, only ginsenoside Rd prolonged the lifespan of to levels comparable to TG. These findings provided mechanistic insight into the antiaging effects of ginsenoside in .
人参是中国东北地区的一种有价值的传统中药。人参中的活性成分——人参皂苷，其抗衰老作用及其潜在的作用机制尚未得到充分研究。在这里，我们研究了总人参皂苷（TG）——从 中提取的主要活性人参皂苷的混合物，对 （）寿命的影响。我们发现 TG 延长了 的寿命并减少了脂褐素的积累。此外，TG 通过减少 ROS 增加了 对热和氧化应激的存活能力。接下来，我们使用 RNA-seq 全面定义 TG 的抗衰老机制。KEGG 通路分析表明，TG 可以延长寿命并参与长寿调节途径。qPCR 显示，TG 上调了 、 、 、 和它们的下游基因的表达。TG 还减少了脂肪积累并促进了脂质代谢。此外，TG 未能延长 和 突变体的寿命，这突出了它们在 TG 抗衰老作用中的作用。然后通过 HPLC 确认了 TG 的四个主要成分，包括人参皂苷 Re、Rg、Rg 和 Rd。在这些人参皂苷中，只有人参皂苷 Rd 能将 的寿命延长到与 TG 相当的水平。这些发现为人参皂苷在 中的抗衰老作用提供了机制上的见解。
