与二甲基亚砜诱导小鼠红白血病细胞向红系分化相关的S期起始的短暂抑制。
Transient inhibition of initiation of S-phase associated with dimethyl sulfoxide induction of murine erythroleukemia cells to erythroid differentiation.
作者信息
Terada M, Fried J, Nudel U, Rifkind R A, Marks P A
出版信息
Proc Natl Acad Sci U S A. 1977 Jan;74(1):248-52. doi: 10.1073/pnas.74.1.248.
Abstract
The murine erythroleukemia cell (MELC) line in suspension culture can be induced to differentiate to erythroid cells by various compounds, including dimethyl sulfoxide (Me2SO). Analysis of the cell cycle, during differentiation induced by Me2SO, using thymidine incorporation, thymidine labeling index, and relative DNA content per cell as measured by flow microfluorometry, demonstrates a transient inhibition of entry of cells into S-phase of the cell cycle which is detected as early as 5 hr and is maximal about 20 hr after beginning of nonsynchronous cultures. Furthermore, in the presence of Me2SO there is restricted binding of the intercalating dye propidium iodide to chromatin from MELC in G1 phase of the cell cycle, as early as 10 hr of culture. This restricted binding of propidium iodide to chromatin is observed in MELC cultured with other inducing agents, such as butyric acid and dimethyl-acetamide, but is not detected with an Me2SO-resistant cell line cultured with Me2SO.
摘要
悬浮培养的小鼠红白血病细胞（MELC）系可被包括二甲基亚砜（Me2SO）在内的多种化合物诱导分化为红细胞。通过使用胸腺嘧啶核苷掺入、胸腺嘧啶核苷标记指数以及通过流式细胞荧光术测量的每个细胞的相对DNA含量，对由Me2SO诱导的分化过程中的细胞周期进行分析，结果表明细胞进入细胞周期S期受到短暂抑制，最早在5小时即可检测到，并且在非同步培养开始后约20小时达到最大值。此外，早在培养10小时时，在细胞周期G1期，在Me2SO存在的情况下，嵌入染料碘化丙啶与MELC染色质的结合受到限制。在用其他诱导剂（如丁酸和二甲基乙酰胺）培养的MELC中也观察到碘化丙啶与染色质的这种结合受限，但在用Me2SO培养的Me2SO抗性细胞系中未检测到这种现象。
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