在弗瑞德红白血病细胞中，G1期的延长与分化并不严格相关。
Lengthening of the G1 phase is not strictly correlated with differentiation in Friend erythroleukemia cells.
作者信息
Friedman E A, Schildkraut C L
出版信息
Proc Natl Acad Sci U S A. 1978 Aug;75(8):3813-7. doi: 10.1073/pnas.75.8.3813.
Abstract
Friend murine erythroleukemia cells (Friend cells) undergo erythroid differentiation in vitro with an increased probability when cells are cultured in the presence of dimethyl sulfoxide (Me2SO) or other agents. Exponentially growing Friend cells, after dilution into medium containing Me2SO, underwent a transient lengthening of the G1 phase of the cell cycle before they became committed to erythroid differentiation. For nine inducing agents, a positive correlation was found between the percentage of cells that had differentiated and synthesized heme, and the percentage of progenitor cells in which a lengthened G1 phase had previously been observed. This correlation was not found, however, with two other potent inducing agents, hypoxanthine and actinomycin D. Moreover, cells that underwent a lengthened G1 phase did not always terminally differentiate. One such example was a Me2SO-resistant, variant Friend cell line (520a) grown in the presence of Me2SO. These results imply that the prolonged G1 phase, although observed with many inducers, is not a prerequisite for erythroid differentiation with all inducers.
摘要
友系小鼠红白血病细胞（Friend细胞）在体外培养时，若在二甲基亚砜（Me2SO）或其他试剂存在的情况下，其发生红系分化的概率会增加。指数生长的Friend细胞在稀释到含有Me2SO的培养基中后，在进入红系分化之前，细胞周期的G1期会出现短暂延长。对于九种诱导剂，已分化并合成血红素的细胞百分比与先前观察到G1期延长的祖细胞百分比之间存在正相关。然而，对于另外两种强效诱导剂次黄嘌呤和放线菌素D，未发现这种相关性。此外，经历G1期延长的细胞并不总是终末分化。一个这样的例子是在Me2SO存在下生长的对Me2SO耐药的变异Friend细胞系（520a）。这些结果表明，尽管许多诱导剂都能观察到G1期延长，但这并非所有诱导剂诱导红系分化的先决条件。
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