Key Lab of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs/Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Engineering Technology Research Center for Fish Breeding and Culture in Hubei Province, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
Hubei Hongshan Laboratory, Wuhan 430070, China.
Int J Mol Sci. 2021 Sep 17;22(18):10056. doi: 10.3390/ijms221810056.
Circular RNAs (circRNAs), a novel class of endogenous RNAs, have been recognized to play important roles in the growth of animals. However, the regulatory mechanism of circRNAs on fish muscle growth is still unclear. In this study, we performed whole transcriptome analysis of skeletal muscles from two populations with different growth rates (fast-growing and slow-growing) of blunt snout bream (), an important fish species for aquaculture. The selected circRNAs were validated by qPCR and Sanger sequencing. Pairs of circRNA-miRNA-mRNA networks were constructed with the predicted differentially expressed (DE) pairs, which revealed regulatory roles in muscle myogenesis and hypertrophy. As a result, a total of 445 circRNAs were identified, including 42 DE circRNAs between fast-growing (FG) and slow-growing (SG) groups. Many of these DE circRNAs were related with aminoglycan biosynthetic and metabolic processes, cytokinetic processes, and the adherens junction pathway. The functional prediction results showed that novel_circ_0001608 and novel_circ_0002886, competing to bind with dre-miR-153b-5p and dre-miR-124-6-5p, might act as competing endogenous RNAs (ceRNAs) to control MamblycephalaGene14755 () and MamblycephalaGene10444 () level, respectively, thus playing an important regulatory role in muscle growth. Overall, these results will not only help us to further understand the novel RNA transcripts in , but also provide new clues to investigate the potential mechanism of circRNAs regulating fish growth and muscle development.
环状 RNA（circRNAs）作为一类新型的内源性 RNA，已被证实对动物的生长发育具有重要作用。然而，circRNAs 对鱼类肌肉生长的调控机制仍不清楚。本研究对生长速度不同（快长型和慢长型）的团头鲂肌肉进行了全转录组分析，团头鲂是一种重要的水产养殖鱼类。通过 qPCR 和 Sanger 测序对筛选出的 circRNAs 进行了验证。利用预测的差异表达（DE）对构建 circRNA-miRNA-mRNA 调控网络，揭示了它们在肌肉发生和肥大中的调控作用。结果共鉴定到 445 个 circRNAs，其中 42 个在快长型（FG）和慢长型（SG）群体之间存在差异表达。这些差异表达的 circRNAs 与氨基糖生物合成和代谢过程、胞质分裂过程以及黏着连接途径有关。功能预测结果表明，novel_circ_0001608 和 novel_circ_0002886 可能作为竞争性内源性 RNA（ceRNA），竞争性结合 dre-miR-153b-5p 和 dre-miR-124-6-5p，分别调控 MamblycephalaGene14755（）和 MamblycephalaGene10444（）的水平，从而在肌肉生长中发挥重要的调控作用。综上所述，这些结果不仅有助于我们进一步了解团头鲂中的新型 RNA 转录本，也为研究 circRNAs 调控鱼类生长和肌肉发育的潜在机制提供了新线索。
