Yamaguchi Yusuke, Matsuno Tomonori, Miyazawa Atsuko, Hashimoto Yoshiya, Satomi Takafumi
Department of Oral and Maxillofacial Surgery, School of Life Dentistry at Tokyo, The Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo 102-8159, Japan.
Oral and Maxillofacial Surgery, The Nippon Dental University Hospital, 2-3-16 Fujimi, Chiyoda-ku, Tokyo 102-8158, Japan.
Materials (Basel). 2021 Sep 8;14(18):5143. doi: 10.3390/ma14185143.
Recently, the frequency of use of bone substitute materials for the purpose of bone augmentation has increased in implant treatment, but bone formation with bone substitute materials alone is limited. Calcification of bone in the body progresses as Ca, HPO, and HPO in the body form hydroxyapatite (HA) crystals. In this study, therefore, we prepared a biphasic bone substitute with biological activity to promote bone formation by inducing precipitation and growth of HA crystals on the surface of a bone substitute and evaluated it. Biphasic bone substitute granules were prepared by immersing HA granules in a supersaturated calcium phosphate solution prepared by mixing five medical infusion solutions, the precipitate was analyzed, and the biological activities of biphasic HA granules were evaluated in vitro and in vivo. As a result, the precipitated calcium phosphate crystals were identified as low crystalline HA. On the surface of the HA granules, low-crystalline HA grew markedly as needle-shaped crystals and significantly promoted cell proliferation and bone differentiation. In animal experiments, biphasic HA granules had a significantly higher bone mineral density, new bone volume ratio, and new bone area ratio. Therefore, it suggests that biphasic hydroxyapatite is a useful bone substitute for bone augmentation in dental implant treatment.
最近，在种植治疗中，用于骨增量目的的骨替代材料的使用频率有所增加，但仅使用骨替代材料的骨形成是有限的。体内骨的钙化过程是随着体内的钙（Ca）、磷酸氢根（HPO）和磷酸二氢根（HPO）形成羟基磷灰石（HA）晶体而进行的。因此，在本研究中，我们制备了一种具有生物活性的双相骨替代材料，通过诱导HA晶体在骨替代材料表面沉淀和生长来促进骨形成，并对其进行了评估。通过将HA颗粒浸入由五种医用输液混合制备的过饱和磷酸钙溶液中来制备双相骨替代颗粒，对沉淀物进行分析，并在体外和体内评估双相HA颗粒的生物活性。结果，沉淀的磷酸钙晶体被鉴定为低结晶度的HA。在HA颗粒表面，低结晶度的HA以针状晶体的形式显著生长，并显著促进细胞增殖和骨分化。在动物实验中，双相HA颗粒的骨矿物质密度、新骨体积比和新骨面积比显著更高。因此，这表明双相羟基磷灰石是牙种植治疗中用于骨增量的一种有用的骨替代材料。
