三种生态评估工具在考察混合多巴胺、血清素、谷氨酸和 GABA 的色谱方法的绿色分析中的应用：比较研究。
Application of Three Ecological Assessment Tools in Examining Chromatographic Methods for the Green Analysis of a Mixture of Dopamine, Serotonin, Glutamate and GABA: A Comparative Study.
机构信息
Department of Pharmaceutical Chemistry, College of Pharmacy, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.
Addiction and Neuroscience Research Unit, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.
出版信息
Molecules. 2021 Sep 7;26(18):5436. doi: 10.3390/molecules26185436.
The assessment of greenness of analytical protocols is of great importance now to preserve the environment. Some studies have analyzed either only the neurotransmitters, dopamine, serotonin, glutamate, and gamma-aminobutyric acid (GABA), together or with other neurotransmitters and biomarkers. However, these methods have not been investigated for their greenness and were not compared with each other to find the optimum one. Therefore, this study aims to compare seven published chromatographic methods that analyzed the four neurotransmitters and their mixtures using the National Environmental Method Index, Analytical Eco-Scale Assessment (ESA), and Green Analytical Procedure Index (GAPI). As these methods cover both qualitative and quantitative aspects, they offer better transparency. Overall, GAPI showed maximum greenness throughout the analysis. Method 6 was proven to be the method of choice for analyzing the mixture, owing to its greenness, according to NEMI, ESA, and GAPI. Additionally, method 6 has a wide scope of application (13 components can be analyzed), high sensitivity (low LOQ values), and fast analysis (low retention times, especially for glutamate and GABA).
现在，评估分析方案的绿色环保程度对于保护环境非常重要。一些研究分析了仅包括神经递质多巴胺、5-羟色胺、谷氨酸和γ-氨基丁酸（GABA），或者是其他神经递质和生物标志物的混合物。然而，这些方法的绿色环保程度尚未得到研究，也没有相互比较以找到最佳方法。因此，本研究旨在使用国家环境方法指数、分析生态规模评估（ESA）和绿色分析程序指数（GAPI）比较七种已发表的用于分析这四种神经递质及其混合物的色谱方法。由于这些方法涵盖了定性和定量方面，因此它们提供了更好的透明度。总的来说，GAPI 在整个分析过程中表现出最大的绿色环保程度。根据 NEMI、ESA 和 GAPI，方法 6 被证明是分析混合物的首选方法，因为它具有绿色环保性。此外，方法 6 具有广泛的应用范围（可分析 13 种成分）、高灵敏度（低 LOQ 值）和快速分析（较短的保留时间，尤其是谷氨酸和 GABA）。
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