Department of Food and Agriculture Production Engineering, University of Rzeszow, St. Zelwerowicza 4, 35-601 Rzeszów, Poland.
Department of Food Technology and Human Nutrition, Rzeszów University, St. Zelwerowicza 4, 35-601 Rzeszów, Poland.
Molecules. 2021 Sep 13;26(18):5548. doi: 10.3390/molecules26185548.
Elderflowers are a well-known source of bioactive compounds. The amount of isolated bioactive compounds may be increased by applying various abiotic and biotic factors. Gaseous ozone (10 and 100 ppm) was used in the process of preparing flowers. Next, the flowers were treated with sugar syrup to extract bioactive compounds. It was shown that this treatment, including the influence of extraction temperature, significantly affects the contents of polyphenols (liquid chromatography-mass spectrometry (LC-MS) methods) and vitamin C, as well as the antioxidant potential (cupric reducing antioxidant capacity (CUPRAC method)), the profile of volatile substances (head space-solid-phase microextraction (HS-SPME methods)) and the colour of the syrup (Commission Internationale de l'Eclairage (CIE) Lab* methods). The findings show that an increased dose of ozone and higher extraction temperature applied in the process of syrup production resulted in higher contents and different compositions of bioactive compounds. The highest contents of bioactive compounds were identified in syrup obtained from raw material treated with ozone for 15 min (concentration = 10 ppm) and extraction with sugar syrup at a temperature of 60 °C.
接骨木花是生物活性化合物的知名来源。通过应用各种非生物和生物因素，可以增加分离的生物活性化合物的含量。在制备鲜花的过程中使用了气态臭氧（10 和 100 ppm）。然后，用糖浆处理鲜花以提取生物活性化合物。结果表明，这种处理，包括提取温度的影响，显著影响多酚（液相色谱-质谱 (LC-MS) 方法）和维生素 C 的含量，以及抗氧化能力（铜还原抗氧化能力 (CUPRAC 方法)）、挥发性物质的特征（顶空固相微萃取 (HS-SPME) 方法）和糖浆的颜色（国际照明委员会 (CIE) Lab* 方法）。研究结果表明，在糖浆生产过程中增加臭氧剂量和提高提取温度会导致生物活性化合物含量增加和组成不同。在 15 分钟臭氧处理（浓度=10 ppm）和 60°C 糖浆提取的原料中获得的糖浆中，鉴定出最高含量的生物活性化合物。
