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提取温度和溶剂类型对罗勒提取物生物活性的影响。

Effect of extraction temperature and solvent type on the bioactive potential of Ocimum gratissimum L. extracts.

机构信息

Bioengineering Department, Faculty of Engineering, Cyprus International University, Northern Cyprus via Mersin 10, 98258, Nicosia, Turkey.

Biotechnology Research Centre, Cyprus International University, Northern Cyprus via Mersin 10, 99258, Nicosia, Turkey.

出版信息

Sci Rep. 2020 Dec 10;10(1):21760. doi: 10.1038/s41598-020-78847-5.

DOI:10.1038/s41598-020-78847-5
PMID:33303935
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7729875/
Abstract

Ocimum gratissimum is a shrub that belongs to the Lamiaceae family of plants. Despite the known biological activities and ethnomedicinal applications, comparative evaluation of the effects of different extraction techniques on the chemical and bioactive properties of O. gratissimum extracts has not yet been performed. This study adopted different analytical techniques to determine the effect of extraction temperature and solvent type on the phytochemical and bioactive properties of O. gratissimum extracts. Chemical profiling showed increased concentrations of compounds for both the ethanolic and methanolic extracts compared to the water extracts. The results also revealed that the extraction temperature had an effect on the total phenolic content and radical-scavenging properties of the different extracts. The antioxidant kinetic modeling achieved the best fit when using the second-order kinetic model. Methanolic extracts had the highest levels of antibacterial activity against Escherichia coli, Bacillus cereus, Staphylococcus aureus, and Salmonella typhimurium. At high concentrations, all extracts lowered the viability of the breast cancer cell line MDA-MB-231. In conclusion, the chemical and bioactive properties of all extracts showed significant dependence on the extraction temperature and solvent type. With proper extraction methods, they boast a wide range of promising applications in the medical, pharmaceutical, and food industries.

摘要

好的，请提供需要翻译的文本。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3267/7729875/a5e9efe82132/41598_2020_78847_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3267/7729875/81a53e1dcb6c/41598_2020_78847_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3267/7729875/e281972c81dd/41598_2020_78847_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3267/7729875/ae44383edff7/41598_2020_78847_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3267/7729875/a5e9efe82132/41598_2020_78847_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3267/7729875/81a53e1dcb6c/41598_2020_78847_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3267/7729875/e281972c81dd/41598_2020_78847_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3267/7729875/ae44383edff7/41598_2020_78847_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3267/7729875/a5e9efe82132/41598_2020_78847_Fig4_HTML.jpg

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