Bang Tae-Hyun, Park Bong-Soo, Kang Hae-Mi, Kim Jung-Han, Kim In-Ryoung
Department of Oral Anatomy, School of Dentistry, Pusan National University, Busandaehak-ro, 49, Mulguem-eup, Yangsan-si 50612, Korea.
Dental and Life Science Institute, School of Dentistry, Pusan National University, Yangsan 50612, Korea.
Pharmaceuticals (Basel). 2021 Sep 5;14(9):902. doi: 10.3390/ph14090902.
Although various methods, such as surgery and chemotherapy, are applied to the treatment of OSCC, there are problems, such as functional and aesthetic limitations of the mouth and face, drug side effects, and lymph node metastasis. Many researchers are making efforts to develop new therapeutic agents from plant-derived substances to overcome the side effects that occur in oral cancer treatment. Polydatin is known as a natural precursor of resveratrol, and research on its efficacy is being actively conducted recently. Therefore, we investigated whether polydatin can induce apoptosis and whether it affects cell migration and invasion through the regulation of EMT-related factors in OSCC. Polydatin decreased the survival and proliferation rates of CAL27 and Ca9-22 cells, and induced the release of cytochrome c, a factor related to apoptosis, and fragmentation of procaspase-3 and PARP. Another form of cell death, autophagy, was observed in polydatin-treated cells. In addition, polydatin inhibits cell migration and invasion, and it has been shown to occur through increased expression of E-cadherin, an EMT related factor, and decreased expression of N-cadherin and Slug and Snail proteins and genes. These findings suggest that polydatin is a potential oral cancer treatment.
尽管诸如手术和化疗等各种方法被应用于口腔鳞状细胞癌（OSCC）的治疗，但仍存在一些问题，如口腔和面部的功能及美观限制、药物副作用以及淋巴结转移。许多研究人员正在努力从植物来源的物质中开发新的治疗药物，以克服口腔癌治疗中出现的副作用。白藜芦醇苷是白藜芦醇的天然前体，最近对其功效的研究正在积极开展。因此，我们研究了白藜芦醇苷是否能诱导细胞凋亡，以及它是否通过调节OSCC中与上皮-间质转化（EMT）相关的因子来影响细胞迁移和侵袭。白藜芦醇苷降低了CAL27和Ca9-22细胞的存活率和增殖率，并诱导了与细胞凋亡相关的细胞色素c的释放，以及半胱天冬酶-3原和聚（ADP-核糖）聚合酶（PARP）的裂解。在白藜芦醇苷处理的细胞中还观察到了另一种形式的细胞死亡——自噬。此外，白藜芦醇苷抑制细胞迁移和侵袭，并且已证明这是通过增加与EMT相关的因子E-钙黏蛋白的表达，以及降低N-钙黏蛋白、Slug和Snail蛋白及基因的表达来实现的。这些发现表明白藜芦醇苷是一种潜在的口腔癌治疗药物。
