Bamphensin Nichari, Chopjitt Peechanika, Hatrongjit Rujirat, Boueroy Parichart, Fittipaldi Nahuel, Gottschalk Marcelo, Kerdsin Anusak
Faculty of Public Health, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000, Thailand.
Department of General Sciences, Faculty of Science and Engineering, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000, Thailand.
Pathogens. 2021 Sep 13;10(9):1178. doi: 10.3390/pathogens10091178.
is a pathogen that causes invasive infections in humans and pigs. In this study, 448 isolates recovered from human infections in Thailand were characterized with regard to their antimicrobial susceptibility and antimicrobial resistance genes, including, for non-penicillin-susceptible isolates, sequence analyses of five genes encoding penicillin-binding proteins (, and ). All 448 isolates were susceptible to cefepime and ceftriaxone, whereas 99.6%, 91.7%, and 72.9% of the isolates were susceptible to levofloxacin, penicillin, and chloramphenicol, respectively. Almost all isolates were resistant to tetracycline (98.2%), clindamycin (94%), erythromycin (92.4%), and azithromycin (82.6%). Genes (O) and were the predominant resistance genes detected among macrolide- and tetracycline-resistant isolates. A total of 37 out of 448 isolates (8.2%) showed intermediately resistance to penicillin. Most of these isolates (59.5%) belonged to serotype 2-ST233. Comparison of the predicted translated sequences of five PBP proteins of a penicillin-susceptible isolate (strain P1/7) to the respective PBP sequences of ten non-penicillin-susceptible isolates revealed multiple amino acid substitutions. Isolates of CC221/234 showed highly variable amino acid substitutions in all PBP proteins. An ST104 isolate had a higher number of amino acid substitutions in PBP2X. Isolates belonging to CC233/379 had numerous substitutions in PBP2B and PBP2X. ST25 isolates exhibited fewer amino acid substitutions than isolates of other STs in all five PBPs. The antimicrobial resistance of is increasing worldwide; therefore, restrictions on antimicrobial use, continuous control, and the surveillance of this bacterium throughout the pork supply chain are crucial for ensuring public health and must be a priority concern.
是一种可导致人类和猪发生侵袭性感染的病原体。在本研究中，对从泰国人类感染中分离出的448株菌株进行了抗菌药物敏感性和抗菌耐药基因特征分析，对于非青霉素敏感菌株，还对编码青霉素结合蛋白的五个基因（、和）进行了序列分析。所有448株菌株对头孢吡肟和头孢曲松敏感，而分别有99.6%、91.7%和72.9%的菌株对左氧氟沙星、青霉素和氯霉素敏感。几乎所有菌株对四环素（98.2%）、克林霉素（94%）、红霉素（92.4%）和阿奇霉素（82.6%）耐药。基因（O）和是在大环内酯类和四环素耐药菌株中检测到的主要耐药基因。448株菌株中共有37株（8.2%）对青霉素呈中介耐药。这些菌株中的大多数（59.5%）属于血清型2-ST233。将一株青霉素敏感菌株（菌株P1/7）的五个PBP蛋白的预测翻译序列与十株非青霉素敏感菌株的相应PBP序列进行比较，发现了多个氨基酸替换。CC221/234菌株在所有PBP蛋白中显示出高度可变的氨基酸替换。一株ST104菌株在PBP2X中有较多的氨基酸替换。属于CC233/379的菌株在PBP2B和PBP2X中有大量替换。ST25菌株在所有五个PBP中显示出比其他ST菌株更少的氨基酸替换。在全球范围内的耐药性正在增加；因此，限制抗菌药物的使用、持续控制以及在整个猪肉供应链中对该细菌进行监测对于确保公众健康至关重要，必须作为优先关注事项。
