从人和猪分离出的猪链球菌中人类相关进化枝标记基因HAC-G19的存在情况。

Presence of Human-Associated Clade Marker Gene HAC-G19 in Streptococcus suis Isolated From Humans and Swine.

作者信息

Hatrongjit Rujirat, Sittichottumrong Kulsatri, Meekhanon Nattakan, Ungcharoen Ratchadaporn, Geng Zou, Boueroy Parichart, Chopjitt Peechanika, Gottschalk Marcelo, Li Jinquan, Kerdsin Anusak

机构信息

Faculty of Science and Engineering, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, 47000, Thailand.

Faculty of Public Health, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, 47000, Thailand.

出版信息

Curr Microbiol. 2025 Jul 21;82(9):400. doi: 10.1007/s00284-025-04387-8.

DOI:10.1007/s00284-025-04387-8

PMID:40690039

Abstract

Streptococcus suis is a zoonotic pathogen contributing to human and pig infections. Effective techniques to distinguish pathogenic from non-pathogenic pathotypes of S. suis is required for public health surveillance. Herein, we developed and evaluated a PCR approach detecting the human-associated clade (HAC)-G19 marker gene to identify the pathogenic pathotype of S. suis. The study was conducted on 599 human strains, 82 diseased-pig strains, and 299 clinically healthy pig strains. PCR analysis revealed that 98.8% and 75.6% were HAC-G19 positive in human and diseased-pig strains, whereas 67.6% of healthy pig strains were negative for the HAC-G19 gene. Statistical analysis revealed that the HAC-G19 gene was more significantly present in diseased-associated isolates than in healthy pig isolates (p-value < 0.001), being with OR of 50.4 (95% CI = 32.1-79.5). Diagnostic accuracy was 87.4% (95% CI = 85.1-89.49%) with sensitivity and specificity were 96.1% (95% CI = 94.3-97.4%) and 67.6% (95% CI = 61.9-72.8%), respectively. This demonstrated that PCR of HAC-G19 has a potential to predict pathogenic pathotype for S. suis.

摘要

猪链球菌是一种人畜共患病原体，可导致人类和猪感染。公共卫生监测需要有效的技术来区分猪链球菌的致病型和非致病型。在此，我们开发并评估了一种检测人类相关进化枝（HAC）-G19标记基因的PCR方法，以鉴定猪链球菌的致病型。该研究对599株人类菌株、82株患病猪菌株和299株临床健康猪菌株进行。PCR分析显示，人类和患病猪菌株中分别有98.8%和75.6%的菌株HAC-G19呈阳性，而67.6%的健康猪菌株HAC-G19基因呈阴性。统计分析显示，与疾病相关的分离株中HAC-G19基因的存在比健康猪分离株更显著（p值<0.001），比值比为50.4（95%置信区间=32.1-79.5）。诊断准确性为87.4%（95%置信区间=85.1-89.49%），敏感性和特异性分别为96.1%（95%置信区间=94.3-97.4%）和67.6%（95%置信区间=61.9-72.8%）。这表明HAC-G19的PCR检测有潜力预测猪链球菌的致病型。