Schenkein H A
J Leukoc Biol. 1986 May;39(5):511-9. doi: 10.1002/jlb.39.5.511.
The human monocytic cell lines HL-60 and U937 were examined for their ability to release PGE and TxB2 in response to the C3 cleavage fragment C3b. It was found that U937 responds to C3b by releasing both eicosanoids only after a 48 h induction period in the presence of PMA. HL-60 failed to respond. The response of U937 was further studied and characterized. It was found that the continual presence of PMA in culture was not required for the response to C3b, and that by replenishing the culture medium every 48 hr, the response could be maintained for up to 12 days. This contrasts with the previously observed behavior of human monocytes, which fail to respond after 24 hr in culture. Further studies demonstrated that maximal levels of PGE and TxB2 were elicited by 25-50 micrograms/ml of C3b, and that the kinetics of appearance of these eicosanoids in culture supernatants was very similar to that previously observed for monocytes. Maximal levels of PGE and TxB2 occurred following 24-48 hr in culture. In addition, a monoclonal a-CR1 antibody stimulated PG release by these cells. It is expected that this cell line will provide a model for study of the influence of cell differentiation on the response of human mononuclear phagocytes to C3b.
检测了人单核细胞系HL-60和U937对C3裂解片段C3b的反应中释放前列腺素E（PGE）和血栓素B2（TxB2）的能力。发现U937仅在存在佛波酯（PMA）的情况下经过48小时诱导期后才通过释放这两种类花生酸对C3b作出反应。HL-60无反应。对U937的反应进行了进一步研究和表征。发现培养中持续存在PMA并非对C3b作出反应所必需，并且通过每48小时补充培养基，反应可持续长达12天。这与人单核细胞先前观察到的行为形成对比，人单核细胞在培养24小时后无反应。进一步研究表明，25 - 50微克/毫升的C3b可引发PGE和TxB2的最高水平，并且这些类花生酸在培养上清液中的出现动力学与先前对单核细胞观察到的非常相似。PGE和TxB2的最高水平在培养24 - 48小时后出现。此外，一种抗补体受体1（CR1）单克隆抗体刺激这些细胞释放前列腺素。预计该细胞系将为研究细胞分化对人单核吞噬细胞对C3b反应的影响提供一个模型。
